1.50032-M
Purospher® STAR RP-8 endcapped (5 µm)
L × I.D. 250 mm x 4.6 mm LiChroCART® HPLC cartridge, manuCART™ holder required for use
Synonym(s):
Purospher® STAR RP-8 endcapped (5µm) LiChroCART® 250-4.6
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About This Item
UNSPSC Code:
41115709
material
stainless steel column
Quality Level
Agency
suitable for USP L7
product line
Purospher®
feature
endcapped
packaging
1 ea of
extent of labeling
11.2% Carbon loading
technique(s)
HPLC: suitable
LC/MS: suitable
column L × I.D.
25 cm × 4.6 mm
surface area
330 m2/g
matrix
fully porous particle
matrix active group
C8 bonding phase
particle size
5 μm
pore size
120 Å pore size
separation technique
reversed phase
storage temp.
no temp limit
General description
Purospher® STAR RP-8 endcapped, 5 µm HPLC columns allow the separation of basic, neutral, and metal chelating compounds with simple mobile phases and excellent peak symmetry. They are based on ultra-pure fully porous silica particles (Type B). These columns offer an outstanding stability from pH 1.5 to 10.5 over a wide temperature range. Purospher® STAR RP-8 endcapped, 5 µm LiChroCART® 250-4.6 is a cartridge column which requires a manuCART™ cartridge column holder part number 1.51486.0001. The manuCART™ can be used to hold one cartridge column with or without direct integration of a 4-4 mm guard column. The recommended guard column is Purospher® STAR RP-8 endcapped (5 µm) LiChroCART® 4-4 part number 1.50270.0001. LiChroCART® cartridge columns mounted with a manuCART™ cartridge column holder is hand-tight only and have therefore a maximum pressure stability of 250 bar.For higher pressure stability requirements Purospher® STAR RP-8 endcapped, 5 µm Hibar® RT 250-4.6 HPLC columns are available part number 1.51454.0001.The column dimension of 250 mm length x 4.6 mm I.D. is the optimal choice for the HPLC separation of complex samples.Item 1.50032.1003 is a Validation kit containing 3 Purospher® STAR RP-8 endcapped, 5 µm LiChroCART® 250-4.6 cartridge columns from 3 different base material lots.Learn more about Purospher® STAR HPLC and UHPLC ColumnsLearn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection GuideBrochure: A Practical Guide to HPLC - Method Development Guide
Application
Intended to be used in the separation of aflatoxins, aldehydes, alkaloids, antibiotics, amino acids, esters, fatty acids, ketones, nitrosamines, nucleosides, PCBs, peptides, phenols, preservatives, steroids, and metabolized steroids.
Features and Benefits
- Ultra-pure silica (99.999%) for excellent peak symmetry
- High separation efficiency
- Reproducible results from run-to-run and batch-to-batch
- Outstanding pH stability from pH 1.5 to 10.5
- Good suitability for LC-MS applications
Analysis Note
Pressure: ≤ 125 bar
Theoretical Plates (N/m) (Toluene): ≥ 85000
Symmetry (Toluene): 0.8 - 1.4
Capacity factor (Toluene): ≥ 1.0
Theoretical Plates (N/m) (Toluene): ≥ 85000
Symmetry (Toluene): 0.8 - 1.4
Capacity factor (Toluene): ≥ 1.0
Legal Information
Hibar is a registered trademark of Merck KGaA, Darmstadt, Germany
LICHROCART is a registered trademark of Merck KGaA, Darmstadt, Germany
MANUCART is a trademark of Merck KGaA, Darmstadt, Germany
Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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