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General description
DLAM-LVproR-13C4 , Kit has 13C4 isotopomer precursors and contains protocol instructions for creation of isotopically-labeled proteins.
Application
DLAM-LVproR-13C4 methyl labeling kit is used for stereospecific labeling of leucine and valine. This kit has been tested with protein isotopic labeling in E. coli. It is used to produce methyl-labeled proteins and acquire precise dynamic information with high resolution in solid-state NMR spectroscopy.
For protein methyl group assignment by 13C isotope labeling of amino acid methyl groups separately or simultaneously.
Packaging
This product may be available from bulk stock and can be packaged on demand. For information on pricing, availability and packaging, please contact Stable Isotopes Customer Service.
Certificates of Analysis (COA)
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Dae-Won Sim et al.
Molecules (Basel, Switzerland), 22(8) (2017-08-16)
A large portion of proteins in living organisms are membrane proteins which play critical roles in the biology of the cell, from maintenance of the biological membrane integrity to communication of cells with their surroundings. To understand their mechanism of
Rime Kerfah et al.
Journal of biomolecular NMR, 63(4), 389-402 (2015-11-15)
A new strategy for the NMR assignment of aliphatic side-chains in large perdeuterated proteins is proposed. It involves an alternative isotopic labeling protocol, the use of an out-and-back (13)C-(13)C TOCSY experiment ((H)C-TOCSY-C-TOCSY-(C)H) and an optimized non-uniform sampling protocol. It has
Silke Wiesner et al.
Current opinion in structural biology, 35, 60-67 (2015-09-26)
Intermolecular interactions are indispensible for biological function. Here we discuss how novel NMR techniques can provide unique insights into the assembly, dynamics and regulation of biomolecular complexes. We focus on applications that exploit the methyl TROSY effect and show that
Articles
Methyl groups are ideal probes for studying protein structure and function by NMR. Choose among kits for isotope labeling of a single type or multiple types of methyl groups.
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