R5628

Hae III from Haemophilus aegyptius

Restriction Enzyme

• CAS Number: 81295-18-3
• Enzyme Commission number: 3.1.21.4 (BRENDA, IUBMB)
• MDL number: MFCD00166568
• UNSPSC Code: 12352204

Properties

• grade: for molecular biology
• form: buffered aqueous glycerol solution
• concentration: 10,000 units/mL
• shipped in: wet ice
• storage temp.: −20°C

Description

Specificity

Recognition sequence: 5′-GG/CC-3′
Ligation and recutting results: After 2-10-fold Hae III overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >50% of fragments can be ligated, and >95% recut.
Heat inactivation: 80 °C for 20 minutes.

Application

HaeIII is a restriction enzyme that is used in molecular biology methods to cleave DNA at the recognition site 5′-GG/CC-3′ to generate DNA fragments with blunt termini.

Other Notes

Supplied with 10x Restriction Enzyme Buffer SM (B3158).

Comment: Inefficient for single-stranded DNA cleavage.
Hae III requires optimal reaction conditions in order to avoid star activity.

Physical form

Solution in 20 mM Tris-HCl, pH 7.7, 0.1 mM EDTA, 400 mM NaCl, 10 mM 2-mercaptoethanol, 50% glycerol (v/v) at 4°C

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable