HPLC Analysis of Triacylglycerols in Olive Oil on Discovery® HS C18 after Sample Prep using Discovery® DSC-Si (Acetone-Acetonitrile Mobile Phase, ELSD Detection)
CONDITIONS
SPE tube/cartridge
Discovery DSC-Si, 1 gm/6 mL (52656-U)
condition
6 mL hexane
sample addition
0.12 g oil in 0.5 mL hexane
elution
10 mL hexane-diethyl ether 87-13 (v/v)
eluate post-treatment
evaporation to dryness, reconstituted into 2 mL acetone
column
Discovery HS C18, 25 cm x 4.6 mm I.D., 5 μm particles (568523-U)
mobile phase
[A] acetone; [B] acetonitrile; (60:40, A:B)
flow rate
1.5 mL/min
pressure
914 psi (63 bar)
column temp.
30 °C
detector
ELSD
injection
5 μL
Description
Analysis Note
Olive oil was pre-cleaned using Silica SPE, analyzed as 5% sample in acetone (w/v).
Legal Information
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany