Skip to Content
Merck

HPLC Analysis of Phenolics from Rosemary on Ascentis® Express C8

HPLC Analysis of Phenolics from Rosemary on Ascentis® Express C8 application for HPLC

Materials

analytical column

Product No.
Description
Pricing

53848-U

Ascentis® Express C8, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 5 cm × 3 mm

standard

Product No.
Description
Pricing

91209

Carnosic acid

analytical standard

49702

Carnosol

analytical standard

CONDITIONS

column

Ascentis Express C8, 5 cm x 3 mm I.D., 2.7 μm particles (53848-U)

mobile phase

[A] 0.1% formic acid in water; [B] 0.1% formic acid in acetonitrile

gradient

15 to 100% B in 8.5 min

flow rate

0.6 mL/min

column temp.

35 °C

detector

UV, 214 nm

injection

2 μL

sample

100 mg/L in 75 μM EDTA, 1.5 mM ascorbate, 15% acetonitrile

Description

Analysis Note

This application demonstrates the suitability of Ascentis Express C8 for the efficient separation of phenolics from rosemary.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Chromatogram Search

Related Product Categories

HPLC ColumnsGC Columns & AccessoriesTLC Plates & AdsorbentsSolid Phase Microextraction (SPME)Solid Phase Extraction & QuEChERS

Related Applications

Small Molecule HPLCLarge Molecule HPLCGas Chromatography (GC)TLCSolid Phase Microextraction (SPME)Solid Phase Extraction (SPE)QuEChERS