HPLC Analysis of BPA (Bisphenol A) at 1 ng/mL in Milk on Ascentis® Express C18 after Solid Phase Extraction using SupelMIP® SPE - Bisphenol A
Materials
analytical column
SPE tube or plate
standard
CONDITIONS
sample preparation
SPE (Solid Phase Extraction)
SPE tube/cartridge
SupelMIP SPE - Bisphenol A, 100 mg/6 mL (54277-U)
sample preparation
(reconstitute residue in 500 μL 40:60 acetonitrile;water)
condition
5 mL methanol 2% acetic acid, 5 mL acetonitrile, 5 mL water (1 drop/sec)
sample addition
5 mL liquid milk, 1 drop/2 sec
drying
-12" vacuum for 120 sec post wash
washing
7 mL water followed by 4 mL 40:60 acetonitrile;water
elution
2 mL methanol
eluate post-treatment
evaporate to dryness at 45 °C under 10 psi nitrogen stream
column
Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.7 μm particles
mobile phase
[A] acetonitrile; [B] water
gradient
40% A held for 3.5 min; to 60% A in 0.1 min; held for 3 min; to 40% A in 0.1 min; held for 13.3 min
flow rate
0.20 mL/min
column temp.
30 °C
detector
FLD, ex 230 nm; em 315 nm
injection
5.0 μL
Description
Analysis Note
Bisphenol A (BPA) is a commonly used synthetic chemical added to a wide variety of plastics and epoxy resins to improve the stability of the final product. BPA has been under scrutiny as an endocrine disruptor, so its level in consumer products, food, and environmental and biological systems is an area of active research and regulation. This application shows a means to purify, concentrate, analyze, and quantify BPA in liquid milk to 1 ng/mL (1 ppb) using SPE and HPLC with fluorescence detection. SupelMIP® SPE - Bisphenol A was used to extract the compound from the milk matrix. Analysis was via HPLC on an Ascentis® Express C18. High purity LC-MS solvents used in the mobile phase provided robust, clean operation.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
SupelMIP is a registered trademark of Sigma-Aldrich Co. LLC