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  • Initiation of DNA replication requires actin dynamics and formin activity.

Initiation of DNA replication requires actin dynamics and formin activity.

The EMBO journal (2017-10-07)
Nikolaos Parisis, Liliana Krasinska, Bethany Harker, Serge Urbach, Michel Rossignol, Alain Camasses, James Dewar, Nathalie Morin, Daniel Fisher
ABSTRACT

Nuclear actin regulates transcriptional programmes in a manner dependent on its levels and polymerisation state. This dynamics is determined by the balance of nucleocytoplasmic shuttling, formin- and redox-dependent filament polymerisation. Here, using

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Monoclonal Anti-PSTAIR antibody produced in mouse, clone PSTAIR, ascites fluid
Sigma-Aldrich
Anti-Actin antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
Sigma-Aldrich
Latrunculin A, from sea sponge, ≥85% (HPLC), waxy solid