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Merck

Avanti lipid tools: connecting lipids, technology, and cell biology.

Biochimica et biophysica acta (2014-06-24)
Kacee H Sims, Ewan M Tytler, John Tipton, Kasey L Hill, Stephen W Burgess, Walter A Shaw
PMID24954118
ABSTRACT

Lipid research is challenging owing to the complexity and diversity of the lipidome. Here we review a set of experimental tools developed for the seasoned lipid researcher, as well as, those who are new to the field of lipid research. Novel tools for probing protein-lipid interactions, applications for lipid binding antibodies, enhanced systems for the cellular delivery of lipids, improved visualization of lipid membranes using gold-labeled lipids, and advances in mass spectrometric analysis techniques will be discussed. Because lipid mediators are known to participate in a host of signal transduction and trafficking pathways within the cell, a comprehensive lipid toolbox that aids the science of lipidomics research is essential to better understand the molecular mechanisms of interactions between cellular components. This article is part of a Special Issue entitled Tools to study lipid functions.

MATERIALS
Product Number
Brand
Product Description
GF88214542
Gold, insulated wire, 1m, conductor diameter 0.05mm, insulation thickness 0.007mm, polyimide insulation, 99.99%
GF43173930
Gold, rod, 25mm, diameter 3.0mm, as drawn, 99.95%
265772
Sigma-Aldrich
Gold, powder, <45 μm, 99.99% trace metals basis
265837
Sigma-Aldrich
Gold, rod, diam. 3.0 mm, 99.99% trace metals basis
310980
Sigma-Aldrich
Gold, wire, diam. 0.5 mm, 99.99% trace metals basis
349240
Sigma-Aldrich
Gold, foil, thickness 0.25 mm, ≥99.9% trace metals basis
349283
Sigma-Aldrich
Gold, wire, diam. 0.1 mm, 99.99% trace metals basis
349275
Sigma-Aldrich
Gold, foil, thickness 0.05 mm, 99.99% trace metals basis
373168
Sigma-Aldrich
Gold, evaporation slug, diam. × L 0.6 cm × 0.6 cm, 99.99% trace metals basis
349305
Sigma-Aldrich
Gold, wire, diam. 1.0 mm, 99.997% trace metals basis
326526
Sigma-Aldrich
Gold, wire, diam. 0.127 mm, 99.99% trace metals basis
326585
Sigma-Aldrich
Gold, powder, <10 μm, ≥99.9% trace metals basis
326534
Sigma-Aldrich
Gold, wire, diam. 0.25 mm, ≥99.9% trace metals basis
326542
Sigma-Aldrich
Gold, beads, 1-6 mm, 99.999% trace metals basis
265829
Sigma-Aldrich
Gold, foil, thickness 0.5 mm, 99.99% trace metals basis
700124P
Avanti
Chol-C10-PC, Avanti Polar Lipids 700124P, powder
GF73794741
Gold, tube, 200mm, outside diameter 2.0mm, inside diameter 1.8mm, wall thickness 0.1mm, as drawn, 99.95%
GF33293729
Gold, insulated wire, 0.1m, conductor diameter 0.125mm, insulation thickness 0.016mm, PTFE (polytetrafluoroethylene) insulation, 99.99%
643289
Sigma-Aldrich
Gold coated glass cover slip, 99.999% (Au), diam. × thickness 15 mm × 130-170 μm, layer thickness 100 Å
643254
Sigma-Aldrich
Gold coated glass cover slip, 99.999% (Au), L × W × thickness 22 mm × 22 mm × 130-170 μm, square, layer thickness 100 Å
636347
Sigma-Aldrich
Gold, nanopowder, <100 nm particle size, 99.9% trace metals basis
643246
Sigma-Aldrich
Gold coated glass slide, layer thickness 1000 Å, 99.999% (Au)
643203
Sigma-Aldrich
Gold coated glass slide, layer thickness 100 Å, 99.999% (Au)
700123P
Avanti
CholPC, Avanti Polar Lipids 700123P, powder
GF31080899
Gold, rod, 25mm, diameter 2.0mm, as drawn, 99.95%
GF34099410
Gold, insulated wire, 2m, conductor diameter 0.125mm, insulation thickness 0.016mm, PTFE (polytetrafluoroethylene) insulation, 99.99%
GF62399133
Gold, insulated wire, 0.5m, conductor diameter 0.05mm, insulation thickness 0.007mm, polyimide insulation, 99.99%
GF20403638
Gold, insulated wire, 1m, conductor diameter 0.125mm, insulation thickness 0.014mm, polyester insulation, 99.99%
GF32175569
Gold, insulated wire, 1m, conductor diameter 0.025mm, insulation thickness 0.005mm, polyester insulation, 99.99%
GF08843867
Gold, rod, 10mm, diameter 3.0mm, as drawn, 99.95%