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Subcellular Fractionation, Enrichment, and Depletion Reagents

Subcellular fractionation techniques

Subcellular fractionation and protein enrichment enable the identification and study of proteins in proteomics research. A wide selection of kits for processing samples from cells, tissues, bacteria, plants, and other sample types is available for subcellular fractionation, protein extraction, depletion, and enrichment.

Organelle Isolation Kits

The biogenesis and maintenance of intracellular organelles is fundamental in cell biology. Establishing and maintaining order requires mechanisms to synthesize and localize proteins to specific organelles and monitor and regulate individual organelles. Targeted proteomics profiling can be partnered with protein pathway function by enriching for organelles and analyzing their contents. These kits and reagents enable researchers to enrich for functional mitochondria, chloroplasts and more with companion kits to determine organelle integrity.

  • Specially formulated extraction reagents and proven procedures suitable for small- or large-scale isolation
  • Isolate intact, functionally active organelles for protein extraction, in vitro assays, and other studies

ProteoExtract® Kits

The ProteoExtract® kits cover the different steps in proteomics sample preparation from protein extraction and abundant protein removal to concentration of protein mixtures, removal of interfering substances, digestion of proteins, selective capturing of phosphorylated peptides, and selective enrichment for specific protein classes. All kits are cross-compatible.

  • Efficient and reproducible protein extraction
  • Protease inhibitor cocktails improve results in downstream analyses
  • Better spot resolution facilitated by nucleic acid digestion with protease-free Benzonase® nuclease
  • Designed for compatibility with many applications including activity assays, Western blots, 1D and 2D PAGE, and mass spectrometry
  • Optimized protocols for different biological samples

ProteoPrep® Extraction and Depletion Kits

ProteoPrep® kits and individual extraction reagents allow for selective or total protein extracts from cellular samples. The protein extractions obtained with each component can be optimized to meet your individual needs. The reducing and alkylating reagents produce protein samples that exhibit improved focusing and decreased streaking in 2D gels. Each kit reagent is also available as an individual product for researchers who have optimized an extraction protocol using one chaotropic extraction reagent.

  • Innovative detergent preparations
  • Premixed solubilization solutions

Related Product Resources

Brochure: Refine Protein Preparation - Tools for Better Protein Analysis

Related Product Categories
Whole Cell Lysis Reagents & Enhancers
Protein extraction kits, cell lysis buffers, and reagents for solubilizing proteins from bacteria, yeast, and insect cultures, as well as plant and mammalian cell cultures and tissue samples.
Protein Mass Spectrometry
To meet your mass spectrometry needs, explore our comprehensive offering of kits, tagging reagents, calibrants, and MS standards.
Phosphatase and Protease Inhibitor Cocktails
Protease and phosphatase inhibitors and cocktails to prevent proteolysis and protein dephosphorylation, and to preserve the active state of proteins during cell lysis, protein extraction, and sample preparation.
Protein Electrophoresis Gels & Buffers
Precast polyacrylamide protein gels, running buffers, and hand-casting reagents for hand-pouring polyacrylamide gels for PAGE and SDS-PAGE protein gel electrophoresis.
Protein Gel Electrophoresis and Transfer Equipment
Electrophoresis tanks, blotting systems, and power supplies for protein gel electrophoresis and wet and semi-dry transfer.
Related Applications
Lysis & Protein Extraction
An overview of cell lysis and protein extraction methods including detergent solubilization, freeze-thaw lysis, osmotic shock, sonication, enzymatic cell lysis, and mechanical disruption techniques such as Dounce, Polytron, and mortar and pestle homogenization.
Protein Purification
Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.
Protein Expression
Protein expression technologies for expressing recombinant proteins in E. coli, insect, yeast, and mammalian expression systems for fundamental research and the support of therapeutics and vaccine production.
Gel Electrophoresis
Protein gel electrophoresis is used to separate proteins for purification, characterization, and detection. Methods such as native PAGE, SDS-PAGE, 2D PAGE, and isoelectric focusing (IEF) are used in preparation for downstream applications including Western blotting, mass spectrometry, and proteomic analysis.
Western Blotting
An overview of the principles and applications of Western blotting for protein detection. Includes links to detailed protocols, videos, and other resources for protein extraction, gel electrophoresis, transfer to PVDF or nitrocellulose membranes, and chemiluminescent, colorimetric, and fluorescent detection methods.
Protein Mass Spectrometry
An overview of techniques for protein mass spectrometry used in the identification and quantification of proteins, post-translational modifications, glycan profiling, and protein-protein interactions in drug discovery and proteomics research.
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