P3391

Protein A-Sepharose^™ from Staphylococcus aureus

lyophilized powder

• Synonym(s): Protein A–Agarose
• MDL number: MFCD00165563
• UNSPSC Code: 41106500
• NACRES: NA.56

Properties

• form: lyophilized powder
• packaging: glass bottle of 1 g; glass bottle of 1.5 g; glass bottle of 250 mg; glass bottle of 5 g
• storage condition: do not freeze
• extent of labeling: 3 mg per mL
• technique(s): affinity chromatography: suitable
• color: white to gray
• matrix: Sepharose CL-4B
• matrix activation: cyanogen bromide
• matrix attachment: amino
• matrix spacer: 1 atom
• capacity: ≥16 mg/mL binding capacity (human IgG)
• solubility: water: 1 g/L at 20 °C
• suitability: conforms to structure for swelling capacity (1g swells to 4 to 5 mL)
• storage temp.: 2-8°C

Description

General description

Protein A-Sepharose when linked with antibody, helps improving antigen binding capacity of IgG crosslinked to column matrices. In this case, binding happens between Protein A and Fc portion of the IgG molecule leaving antigen specific sites free.

Application

Protein A-Sepharose was used:

• for immunoprecipitated placentas for protein and RNA analyses using Western blot technique.
• in chromatin immunoprecipitation to understand the link between Mediator and remodelling of chromatin structure at specific promoters.
• as lysis buffer to remove endogenous G-type Igs in protein extraction of brain samples from female rats.
• in immunoprecipitation of [³²P] phosphomyristin C of rabbit antiserum, to study the down-regulation of protein kinase C (PKC) and phosphomyristin C (PMC) during T-cell development.

Protein A-Sepharose^™ is used for affinity chromatography, antibody purification and characterization, immunoaffinity matrices, protein chromatography, protein A, G and L resins, and recombinant protein expression and analysis. Protein A-Sepharose^™ has been used to develop strategies for investigating protein interactions, to improve the detection of celiac disease, and to study diabetes in children.

Quantity

Swelling: 1 g swells to approx. 4 ml.

Physical form

Supplied as lyophilized powder stabilized with lactose and dextran.

Legal Information

Sepharose is a trademark of Cytiva

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves