MABC1142

Anti-TIMP-4 Antibody, clone 9:4-7

clone 4-7, from mouse

• Synonym(s): Tissue inhibitor of metalloproteinases 4, Metalloproteinase inhibitor 4
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 4-7, monoclonal
• species reactivity: human
• packaging: antibody small pack of 25 μL
• technique(s): ELISA: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• isotype: IgG2bκ
• NCBI accession no.: NP_003247.1
• UniProt accession no.: Q99727
• target post-translational modification: unmodified
• Gene Information: human ... TIMP4(7079)

Description

General description

Tissue inhibitor of metalloproteinases 4 (UniProt: Q99727; also known as Metalloproteinase inhibitor 4, TIMP-4) is encoded by the TIMP4 gene (Gene ID: 7079) in human. The tissue inhibitors of metalloproteinases (TIMPs) represent a family of ubiquitous proteins which are natural inhibitors of the matrix metalloproteinases (MMPs). There are four known members of the TIMP family that are designated as TIMP-1, 2, 3, and 4. TIMP-4 is synthesized with a signal peptide (aa 1-29), which is cleaved off in the mature form. It has two MMP-binding regions (aa 30-33 and 99-100). TIMP-4 expressed is observed in heart, brain, pancreas, kidney, colon, testes, and adipose tissue. However, elevated levels of TIMP-4 have been reported in various cancers tissues, including breast, ovary, prostate, colon and kidney and are associated with poor survival prognosis. Hence, it can serve as a useful marker for disease progression. TIMP-4 is shown to complex MMPs and irreversibly inactivates them by binding to their catalytic zinc cofactor. This clone (9:4 7) is specific for human TIMP-4 and does not exhibit any cross-reactivity to other TIMPs. (Ref.: Gomez, DE., et al. (1997). Eur. J. Cell. Biol. 74(2); 111-122; Donover, PS et AL (2010). J. Cell. Biochem. 110(5); 1255-1261).

Specificity

Clone 9:4-7 specifically detects human TIMP-4.

Immunogen

GST-tagged full-length recombinant human TIMP-4.

Application

Anti-TIMP-4, clone 9:4-7, Cat. No. MABC1142, is a mouse monoclonal antibody that detects Tissue Inhibitor of Matrix Metalloproteinase 4 and has been tested for use in ELISA, Immunohistochemistry (Paraffin), and Western Blotting.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected TIMP-4 in human kidney tissue sections.

Immunohistochemistry (Paraffin) Analysis: A representative lot detected TIMP-4 in Immunohistochemistry applications (Donover, P.S., et. al. (2010). J Cell Biochem. 110(5):1255-61).

ELISA Analysis: A representative lot detected TIMP-4 in ELISA applications (Donover, P.S., et. al. (2010). J Cell Biochem. 110(5):1255-61).

Western Blotting Analysis: A representative lot detected TIMP-4 in Western Blotting applications (Donover, P.S., et. al. (2010). J Cell Biochem. 110(5):1255-61).

Research Category
Apoptosis & Cancer

Quality

Evaluated by Immunohistochemistry (Paraffin) in human breast cancer tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected TIMP-4 in human breast cancer tissue sections.

Target description

25.50 kDa calculated.

Physical form

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable