ABT155

Anti-Dynamin related protein 1 (Drp1) Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Dynamin-1-like protein, Dynamin family member proline-rich carboxyl-terminal domain less, Dymple, Dynamin-related protein 1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human, mouse
• species reactivity (predicted by homology): rhesus macaque (based on 100% sequence homology), rat (based on 100% sequence homology), ox (based on 100% sequence homology)
• technique(s): immunocytochemistry: suitable; western blot: suitable
• NCBI accession no.: NP_690029
• UniProt accession no.: Q8K1M6
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: mouse ... Dnm1L(74006)

Description

General description

Dynamin related protein 1 (Drp1), also known as Dynamin-like protein 1, mediates outter mitochondrial membrane fission in mammilian cells. Like other members of the Dynamin superfamily of GTPases, Drp1 contains a N-terminal tripartate GTP-binding domain and exists as a T-shaped dimer with a head, leg, and stalk. The addition of GTP induces head and stalk rearrangement resulting in membrane constriction. Drp1 has been shown to affect mitochondrial morphology and places a significant role in mitochondrial and peroxisomal fission in mammilian cells. During apoptosis, Drp1 translocates from the cytosol to mitochondria and localizes to potential organelle division sites. Studies show Drp1 inhibition averts cell death, reinforcing to the critical role that mitochondrial fission plays in apoptosis.

Specificity

This antibody recognizes Dynamin related protein 1 (Drp1) at the GSK3B interaction domain.

Immunogen

Epitope: GSK3B interaction domain

KLH-conjugated linear peptide corresponding to the GSK3B interaction domain of mouse Dynamin related protein 1 (Drp1).

Application

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Dynamin related protein 1 in human embryonic stem cell H9 cells.

Research Sub Category
Organelle & Cell Markers

Apoptosis - Additional

This Anti-Dynamin related protein 1 (Drp1) Antibody is validated for use in WB, IC for the detection of Dynamin related protein 1 (Drp1).

Quality

Evaluated by Western Blot in mouse embryonic stem cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Dynamin related protein 1 (Drp1) on 10 µg of mouse embryonic stem cell lysate.

Target description

~80kDa (isoform 2) & ~82 kDa (isoform 1) observed
5 isoforms are produced by alternative splicing: Isoform 1: 80 kDa, Isoform 2: 82 kDa, Isoform 3: 78 kDa, Isoform 4: 69 kDa, and Isoform 5: 24 kDa

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable