SnapFast™ Cloning & Expression Vectors Selection Charts

With over 1600 vectors to choose from, your options may seem endless. For help choosing the right vector for your research needs, consult the tables below.

Bacterial vector options with FLAG and 3xFLAG tags

Product Number	FLAG tag		OmpA secretion tag	Ampicillin Selection Marker	EKT cleavage tag	Promoter
	N-terminal	C-terminal				Inducible	TAC	T7 Lac
OGS632	x			x	x	x	x
OGS632	x		x	x	x	x	x
OGS634		x		x	x	x	x
OGS635		x	x	x	x	x	x
OGS636	x			x	x	x		x

Mammalian vector options with FLAG and 3xFLAG tags

Product Number	FLAG tag		3X FLAG tag		C-myc tag	EKT cleavage tag	PPT secretion tag	Ampicillin Selection Marker (Bacterial)	Neomycin Selection Marker (Mammalian)	Promoter
	N-terminal	C-terminal	N-terminal	C-terminal	C-terminal					Constitutive	CMV
OGS618	x					x		x		x	x
OGS619		x				x		x		x	x
OGS620			x			x		x		x	x
OGS621			x			x	x	x		x	x
OGS622			x		x	x	x	x		x	x
OGS623			x		x	x		x		x	x
OGS624	x					x	x	x	x	x	x
OGS625	x					x		x	x	x	x
OGS626			x			x	x	x	x	x	x
OGS627			x			x		x	x	x	x
OGS628				x		x	x	x	x	x	x
OGS629				x		x		x	x	x	x
OGS630			x		x	x	x	x	x	x	x
OGS631			x		x	x		x	x	x	x

Vector options for secretory tags for use in bacterial cells

Secretory Tag: OmpA		Functional Tag
		T7	V5	FLAG®	Strep	c-myc	6-His	HA	S-tag
Cleavage Site	EKT	OGS3073	OGS3049	OGS3067	OGS3085	OGS3055	OGS3043	OGS3061	OGS3079
	Thr	OGS3075	OGS3051	OGS3069	OGS3087	OGS3057	OGS3045	OGS3063	OGS3081
	Fxa	OGS3076	OGS3052	OGS3070	OGS3088	OGS3058	OGS3046	OGS3064	OGS3082
	TEV	OGS3072	OGS3048	OGS3066	OGS3084	OGS3054	OGS3042	OGS3060	OGS3078
	3C	OGS3077	OGS3053	OGS3071	OGS3089	OGS3059	OGS3047	OGS3065	OGS3083
	none	OGS3095	OGS3092	OGS3098	OGS3097	OGS3093	OGS3090	OGS3094	OGS3096
Secretory Tag: PelB
Cleavage Site	EKT	OGS3131	OGS3107	OGS3125	OGS3143	OGS3113	OGS3101	OGS3119	OGS3137
	Thr	OGS3133	OGS3109	OGS3127	OGS3145	OGS3115	OGS3103	OGS3121	OGS3139
	Fxa	OGS3134	OGS3110	OGS3128	OGS3146	OGS3116	OGS3104	OGS3122	OGS3140
	TEV	OGS3130	OGS3106	OGS3124	OGS3142	OGS3112	OGS3100	OGS3118	OGS3136
	3C	OGS3135	OGS3111	OGS3129	OGS3147	OGS3117	OGS3105	OGS3123	OGS3141
	none	OGS3153	OGS3150	OGS3156	OGS3155	OGS3151	OGS3148	OGS3152	OGS3154

Vector options for secretory tags for use in yeast cells

Secretory Tag: Alpha amylase		Functional Tag
		T7	V5	FLAG®	Strep	c-myc	6-His	HA	S-tag
Cleavage Site	EKT	OGS1791	OGS1767	OGS1785	OGS1803	OGS1773	OGS1761	OGS1779	OGS1797
	Thr	OGS1793	OGS1769	OGS1787	OGS1805	OGS1775	OGS1763	OGS1781	OGS1799
	Fxa	OGS1794	OGS1770	OGS1788	OGS1806	OGS1776	OGS1764	OGS1782	OGS1800
	TEV	OGS1790	OGS1766	OGS1784	OGS1802	OGS1772	OGS1760	OGS1778	OGS1796
	3C	OGS1795	OGS1771	OGS1789	OGS1807	OGS1777	OGS1765	OGS1783	OGS1801
	none	OGS1813	OGS1810	OGS1816	OGS1815	OGS1811	OGS1808	OGS1812	OGS1814
Secretory Tag: Alpha Factor (full length)
Cleavage Site	EKT	OGS1849	OGS1825	OGS1843	OGS1861	OGS1831	OGS1819	OGS1837	OGS1855
	Thr	OGS1851	OGS1827	OGS1845	OGS1863	OGS1833	OGS1821	OGS1839	OGS1857
	Fxa	OGS1852	OGS1828	OGS1846	OGS1864	OGS1834	OGS1822	OGS1840	OGS1858
	TEV	OGS1848	OGS1824	OGS1842	OGS1860	OGS1830	OGS1818	OGS1836	OGS1854
	3C	OGS1853	OGS1829	OGS1847	OGS1865	OGS1835	OGS1823	OGS1841	OGS1859
	none	OGS1871	OGS1868	OGS1874	OGS1873	OGS1869	OGS1866	OGS1859	OGS1872

Vector options for secretory tags for use in mammalian cells

Secretory Tag: insulin		Functional Tag
		T7	V5	FLAG®	Strep	c-myc	6-His	HA	S-tag
Cleavage Site	EKT	OGS1410	OGS1386	OGS1404	OGS1422	OGS1392	OGS1380	OGS1398	OGS1416
	Thr	OGS1412	OGS1388	OGS1406	OGS1424	OGS1394	OGS1382	OGS1400	OGS1418
	Fxa	OGS1413	OGS1389	OGS1407	OGS1425	OGS1395	OGS1383	OGS1401	OGS1419
	TEV	OGS1409	OGS1385	OGS1403	OGS1421	OGS1391	OGS1379	OGS1397	OGS1415
	3C	OGS1414	OGS1390	OGS1408	OGS1426	OGS1396	OGS1384	OGS1402	OGS1420
	none	OGS1432	OGS1429	OGS1435	OGS1434	OGS1430	OGS1427	OGS1431	OGS1433

Secretory Tag: BM40
Cleavage Site	EKT	OGS1468	OGS1444	OGS1462	OGS1480	OGS1450	OGS1438	OGS1456	OGS1474
	Thr	OGS1470	OGS1446	OGS1464	OGS1482	OGS1452	OGS1440	OGS1458	OGS1476
	Fxa	OGS1471	OGS1447	OGS1465	OGS1483	OGS1453	OGS1441	OGS1459	OGS1477
	TEV	OGS1467	OGS1443	OGS1461	OGS1479	OGS1449	OGS1437	OGS1455	OGS1473
	3C	OGS1472	OGS1448	OGS1466	OGS1484	OGS1454	OGS1442	OGS1460	OGS1478
	none	OGS1490	OGS1487	OGS1493	OGS1492	OGS1488	OGS1485	OGS1489	OGS1491

Vector options for single functional tags for use in yeast cells

Yeast Plasmids Encoding Single Functional Tags

N Terminal Tag Plasmids are in Blue
C Terminal Tag Plasmids are in Red

		Protease Cleavage Site
		none	EKT	TEV	FXa	Throm	3C
Epitope Tags	V5	OGS3247 OGS3457	OGS3237 OGS3446	OGS3228 OGS3438	OGS3225 OGS3435	OGS3226 OGS3436	OGS3230 OGS3440
	Ha	OGS3250 OGS3460	OGS3235 OGS3444	OGS1634 OGS1917	OGS1604 OGS1887	OGS1614 OGS1897	OGS1643 OGS1926
	C-Myc	OGS3249 OGS3459	OGS3234 OGS3443	OGS1633 OGS1916	OGS1603 OGS1886	OGS1613 OGS1896	OGS1642 OGS1925
	S-Tag	OGS3254 OGS3464	OGS1652 OGS1935	OGS1638 OGS1921	OGS1608 OGS1891	OGS1618 OGS1901	OGS1647 OGS1930
	T7	OGS3253 OGS3463	OGS1651 OGS1934	OGS1637 OGS1920	OGS1607 OGS1890	OGS1617 OGS1900	OGS1646 OGS1929
	FLAG®	OGS3248 OGS3458	OGS3232 OGS3442	OGS1632 OGS1915	OGS1602 OGS1885	OGS1612 OGS1895	OGS1641 OGS1924
Affinity Tags & Solubility Tags	MBP	OGS3255 OGS3465	OGS1653 OGS1936	OGS3229 OGS3439	OGS1609 OGS1892	OGS1619 OGS1902	OGS1648 OGS1931
	GST	OGS3251 OGS3461	OGS3236 OGS3445	OGS1635 OGS1918	OGS1605 OGS1888	OGS1615 OGS1898	OGS1644 OGS1917
	Strep	OGS3252 OGS3462	OGS1650 OGS1933	OGS1636 OGS1919	OGS1606 OGS1889	OGS1616 OGS1899	OGS1645 OGS1928
	6His	OGS3246 OGS3456	OGS1631 OGS1914	OGS1601 OGS1884	OGS1601 OGS1884	OGS1611 OGS1894	OGS1640 OGS1923
	10His	OGS3245 OGS3455	OGS1469 OGS1932	OGS1630 OGS1913	OGS1600 OGS1883	OGS1610 OGS1893	OGS1639 OGS1922

Vector options for dual functional tags for use in yeast cells

Yeast Plasmids Encoding Dual Functional Tags

N Terminal Tag Plasmids are in Blue
C Terminal Tag Plasmids are in Red

		Enzyme Cleavage Site
Dual tags		EKT	TEV	FXa	Thrombin	3C
	6His & V5	OGS3238 OGS3244 OGS3447 OGS3454	OGS3242 OGS3452	OGS3239 OGS3448	OGS3240 OGS3450	OGS3243 OGS3453
	6His & GST	OGS1661 OGS1705 OGS1944 OGS1988	OGS1689 OGS1972	OGS1665 OGS1948	OGS1673 OGS1956	OGS1697 OGS1980
	6His & HA	OGS1660 OGS1704 OGS1943 OGS1987	OGS1688 OGS1971	OGS1664 OGS1664	OGS1672 OGS1955	OGS1696 OGS1979
	6His & C-Myc	OGS1659 OGS1703 OGS1942 OGS1986	OGS1687 OGS1970	OGS1663 OGS1946	OGS1671 OGS1954	OGS1695 OGS1978
	6His & S-Tag	OGS1656 OGS1708 OGS1939 OGS1991	OGS1692 OGS1975	OGS1668 OGS1951	OGS1676 OGS1959	OGS1700 OGS1983
	6His & T7	OGS1655 OGS1707 OGS1938 OGS1990	OGS1691 OGS1974	OGS1667 OGS1950	OGS1675 OGS1958	OGS1699 OGS1982
	6His & FLAG	OGS1658 OGS1702 OGS1941 OGS1985	OGS1686 OGS1969	OGS1662 OGS1945	OGS1670 OGS1953	OGS1694 OGS1977
	6His & MBP	OGS1657 OGS1709 OGS1940 OGS1992	OGS1693 OGS1976	OGS1669 OGS3449	OGS1677 OGS1960	OGS1701 OGS1984
	6His & Strep	OGS1654 OGS1706 OGS1937 OGS1969	OGS1690 OGS1973	OGS1666 OGS1949	OGS1674 OGS1957	OGS1698 OGS1981

Vector options for fluorescent reporters

Fluorescence Reporter Gene Plasmids

				GFP (DaGFP)	YFP (KrYFP)	CFP (FrCFP)
Single Gene Vectors	Choose a promoter to drive the reporter gene	CMV Promoter		OGS242	OGS510	OGS509
		Minimal CMV Promoter		OGS573	OGS574	OGS575
	Systems for you to insert your own promoter	With polyA upstream of MCS - to minimise background	Prom-MCS-pAa	OGS241	OGS614	OGS615
			MinProm-pAb	OGS251	–	–
			CMVe-pAc	OGS257	–	–
		Without upstream pA - better for cloning into retrovirus or lentivirus	Prom MCS (no pA)a	OGS347	–	–
			MinProm (no pA)b	OGS362	–	–
			CMVe (no pA)c	OGS368	–	–
Double Gene Vectors (place your gene of interest into the MCS, under CMV promoter control)	Chose a promoter to drive the reporter gene	RSV		OGS243	OGS516	OGS515
		Ubiquitin			OGS513	OGS512
		PGK		OGS390	OGS611	OGS610
		IRES (Internal Ribosome Entry Site)	FMDV	OGS289	OGS521	OGS522
			EMCV	OGS315	OGS518	OGS519
Reporter Fusion Protein Contructs		Separate table

NOTES:

(a)PromMCS plasmids have no promoter driving the reporter gene, but they have several unique restriction sites that can be used to insert the promoter of your choice.

(b)MinProm plasmids contain the minimal HSV TK promoter driving the reporter gene, with an upstream MCS that can be used to introduce specificity-defining sequences (such as transcription factor binding sites) of your choice

(c)CMVe plasmids contain the CMV enhancer upstream of the MCS, for your to insert your promoter of choice. The CMV enhancer should augment its activity.

Vector options for fusion reporters

Mammalian Reporter Fusion Protein Plasmids

N Terminal Tag Plasmids are in Blue
C Terminal Tag Plasmids are in Red

Tag	Enzyme for removal	Luciferase			DaGFP	KrYFP	FrCFP	Beta gal	Cat	SEAP
		Firefly	Renilla	iLumena (secreted)
None	–	OGS1371 OGS1372	OGS1369 OGS1370	OGS1378	OGS1363 OGS1364	OGS1367 OGS1368	OGS1365 OGS1366	OGS1373 OGS1374	OGS1375 OGS1376	OGS1377
6His	EKT	OGS1287 OGS1288	OGS1285 OGS1286		OGS1279 OGS1280	OGS1283 OGS1284	OGS1281 OGS1282	OGS1289 OGS1290	OGS1291 OGS1292
	TEV	OGS1343 OGS1344	OGS1341 OGS1342		OGS1335 OGS1336	OGS1339 OGS1340	OGS1337 OGS1338	OGS1345 OGS1346	OGS1347 OGS1348
	FXa	OGS1301 OGS1302	OGS1299 OGS1300		OGS1293 OGS1294	OGS1297 OGS1298	OGS1295 OGS1296	OGS1303 OGS1304	OGS1305 OGS1306
	Thr	OGS1315 OGS1316	OGS1313 OGS1314		OGS1307 OGS1308	OGS1311 OGS1312	OGS1309 OGS1310	OGS1317 OGS1318	OGS1319 OGS1320
	3C	OGS1357 OGS1358	OGS1355 OGS1356		OGS1349 OGS1350	OGS1353 OGS1354	OGS1351 OGS1352	OGS1359 OGS1360	OGS1361 OGS1362

Vector options for the Luciferase reporter

Luciferase Reporter Gene Plasmids

				Firefly Luc (cytosolic)	Renilla Luc (cytosolic)	iLumena Luc (secreted)
Single Gene Vectors	Choose a promoter to drive the reporter gene	CMV Promoter		OGS102	OGS103	OGS511 OGS1378 (puro)
		Minimal CMV Promoter		OGS570	OGS571	OGS578
		Other Promoters		Chicken beta actin OGS598	CAG OGS508
	Systems for you to insert your own promoter	With polyA upstream of MCS - to minimise background	Prom-MCS-pAa	OGS236	OGS237	OGS616
			MinProm-pAb	OGS246	OGS247
			CMVe-pAc	OGS252	OGS253
		Without upstream pA - better for cloning into retrovirus or lentivirus	Prom MCS (no pA)a	OGS369	OGS370
			MinProm (no pA)b	OGS357	OGS358
			CMVe (no pA)c	OGS363	OGS364
	Chose a promoter to drive the reporter gene	CMV		OGS608
Double Gene Vectors (place your gene of interest into the MCS, under CMV promoter control)		RSV		OGS15	OGS16	OGS517
		Ubiquitin		OGS155	OGS156	OGS514
		PGK		OGS388	OGS389	OGS609
		IRES (Internal Ribosome Entry Site)	FMDV	OGS285	OGS286	OGS523
			EMCV	OGS295 OGS602 (puro)	OGS296	OGS520
Reporter Fusion Protein Contructs		Separate table

NOTES:

(a)PromMCS plasmids have no promoter driving the reporter gene, but they have several unique restriction sites that can be used to insert the promoter of your choice.

(b)MinProm plasmids contain the minimal HSV TK promoter driving the reporter gene, with an upstream MCS that can be used to introduce specificity-defining sequences (such as transcription factor binding sites) of your choice

(c)CMVe plasmids contain the CMV enhancer upstream of the MCS, for your to insert your promoter of choice. The CMV enhancer should augment its activity.

Vector options for Colorimetric reporters

Colorimetric Reporter Gene Plasmids

				Alkaline phosphatase SEAP (secreted)	Beta galactosidase (cytosolic)	CAT Chloramphenicol Acetyl Transferase (cytosolic)
Single Gene Vectors	Choose a promoter to drive the reporter gene	CMV Promoter		OGS105	OGS101 OGS594 (+Puro)	OGS104
		Minimal CMV Promoter		OGS577	OGS572	OGS576
		Other Promoters			CAG OGS506 (+Puro)
	Systems for you to insert your own promoter	With polyA upstream of MCS - to minimise background	Prom-MCS-pAa	OGS238	OGS239	OGS240
			MinProm-pAb	OGS248	OGS249	OGS250
			CMVe-pAc	OGS254	OGS255	OGS256
		Without upstream pA - better for cloning into retrovirus or lentivirus	Prom MCS (no pA)a	OGS371	OGS372	OGS373
			MinProm (no pA)b	OGS359	OGS360	OGS361
			CMVe (no pA)c	OGS365	OGS366	OGS367
Double Gene Vectors (place your gene of interest into the MCS, under CMV promoter control)	Chose a promoter to drive the reporter gene	RSV		OGS17	OGS18	OGS19
		Ubiquitin		OGS157	OGS158	OGS159
		PGK		OGS392	OGS391	OGS393
		IRES (Internal Ribosome Entry Site)	FMDV	OGS287
			EMCV
Reporter Fusion Protein Contructs		Separate table

NOTES:

(a)PromMCS plasmids have no promoter driving the reporter gene, but they have several unique restriction sites that can be used to insert the promoter of your choice.

(b)MinProm plasmids contain the minimal HSV TK promoter driving the reporter gene, with an upstream MCS that can be used to introduce specificity-defining sequences (such as transcription factor binding sites) of your choice

(c)CMVe plasmids contain the CMV enhancer upstream of the MCS, for your to insert your promoter of choice. The CMV enhancer should augment its activity.

Looking for more vector options to move your experiments forward faster? Consider a custom cloning vector designed and built by Oxford Genetics™. Find out more at Oxford Genetics - our partner for cloning and expression vectors for molecular biology and synthetic biology applications.