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LC/MS Analysis of Drugs of Abuse on Ascentis® Express RP-Amide

LC/MS Analysis of Drugs of Abuse on Ascentis® Express RP-Amide application for HPLC

Materials

analytical column

Product No.
Description
Pricing

53913-U

Ascentis® Express RP-Amide, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 10 cm × 2.1 mm

mobile phase component

Product No.
Description
Pricing

70221

Ammonium formate

eluent additive for LC-MS, LiChropur™, ≥99.0%

standard

Product No.
Description
Pricing

B-007

Benzoylecgonine solution

100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

C-010

Cocaethylene solution

1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®

E-006

EDDP perchlorate

ampule of 10 mg, certified reference material, Cerilliant®

CONDITIONS

column

Ascentis Express RP Amide, 10 cm x 2.1 mm I.D., 2.7 μm particles (53913-U)

mobile phase

[A] 10 mM ammonium formate in water; [B] 10mM ammonium formate in acetonitrile; (75:25, A:B)

flow rate

0.2 mL/min

column temp.

35 °C

detector

ESI(+)

injection

2 μL

sample

500 ng/mL in water

Description

Analysis Note

This applic.ation demonstrates the suitability of Ascentis Express RP-Amide for the efficient separation of common drink additives used in diet cola

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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