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  • Studies toward the site specific incorporation of sugars into proteins: synthesis of glycosylated aminoacyl-tRNAs.

Studies toward the site specific incorporation of sugars into proteins: synthesis of glycosylated aminoacyl-tRNAs.

Carbohydrate research (2001-02-24)
N E Fahmi, S Golovine, B Wang, S M Hecht
ABSTRACT

A series of glycosylated serine derivatives was synthesized from peracetylated sugars and Fmoc-protected serine; these were chemically esterified with the tris-(tetrabutylammonium) salt of pdCpA. The fully protected and deprotected glycosylated aminoacyl pdCpAs were ligated enzymatically to an abbreviated tRNA (tRNA-C(OH)) to provide the title compounds that are key intermediates in the elaboration of glycoproteins using readthrough of a nonsense codon.

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Dansylhydrazine, BioReagent, suitable for fluorescence, ≥90% (HPLC)
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Sigma-Aldrich
Dansylhydrazine, 98%
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