- Novel minimum ribozymes with oxidoreduction activity: 5-hydroxyuridine, 8-hydroxyguanosine, and 8-hydroxyadenosine isolated from Torula yeast RNA.
Novel minimum ribozymes with oxidoreduction activity: 5-hydroxyuridine, 8-hydroxyguanosine, and 8-hydroxyadenosine isolated from Torula yeast RNA.
Nucleic acids symposium series (1991-01-01)
H Yanagawa, Y Ogawa, M Ueno
PMID1842045
ABSTRACT
Three nucleosides catalyzing the oxidoreduction of NADH and K3Fe(CN)6 were isolated from Torula yeast RNA and also obtained by a series of steps: SDS-phenol extraction, nuclease P1 digestion, alkaline phosphatase digestion, anion exchange chromatography, and HPLC on an ODS column. Their chemical structures were clearly determined as 5-hydroxyuridine, 8-hydroxyguanosine, and 8-hydroxyadenosine from the results of FAB-MS, 1H and 13C-NMR spectroscopies.