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Amicon® Devices

Amicon® Ultra centrifugal devices for protein concentration, desalting, and buffer exchange

Amicon® ultrafiltration devices provide a fast, reliable method to concentrate proteins and other biomolecules, separate components in biological and environmental samples by molecular weight, and desalt and dialyze proteins and antibodies for sample preparation.

Ultrafiltration (UF) gently separates molecules based on molecular weight, using pressure to drive solutes and molecules through a semipermeable filter membrane. The nominal molecular weight limit (NMWL) of the filter membrane defines the particle size cut-off where >90% of particulate is retained. Solutes larger than the specified NMWL will remain upstream of the filter in the retentate, while water and solutes smaller than the specified NMWL pass through the membrane into the filtrate. This allows concentration of samples through the removal of excess liquid; buffer exchange and desalting by replacement of aqueous buffer; and separation, enrichment, and removal of macromolecules by size for sample preparation.

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Amicon® Ultra Centrifugal Filter Devices

Amicon® Ultra centrifugal filters enable rapid concentration, desalting, dialysis, and diafiltration of 0.5 mL -15 mL sample volumes with high sample recovery. Amicon® Ultra centrifugal devices use Ultracel® regenerated cellulose (RC) membranes for filtration, offered with nominal molecular weight limits (NMWLs) of 3 kDa, 10 kDa, 30 kDa, 50 kDa, and 100 kDa.

Features:

  • Small- and mid-volume ultrafiltration: available for 0.5 mL - 15 mL sample volumes
  • High recovery: low binding membrane and polypropylene housing for >90% sample recovery
  • Quick processing times: ultra-fast sample processing

in as little as 10 minutes

  • Rapid protein concentration: 25- to 80-fold concentration in a single step
  • Broad chemical compatibility: compatible with a variety of aqueous solutions, pH 1 to 9
  • Consistent and reliable sample prep: predictable concentration factors, with no drying out of sample
  • Reverse spin capability: maximum protein recovery, even with small, dilute samples

Amicon® Pro Adapters

Amicon® Pro Adapters are designed for use with Amicon® Ultra centrifugal filtration devices to enable multiple simultaneous protein workflow processes including, but not limited to, dialysis, diafiltration, affinity purification, buffer exchange, filter-aided sample preparation (FASP), and concentration. The Amicon® Pro Adapter is the only product on the market that offers the flexibility to perform any or all protein workflow manipulations in a single device for maximum recovery without sample transfer. The Amicon® Pro Adapter is comprised of a 10 mL chamber for wash and buffer exchange steps, a frit that allows the insertion of affinity resins and simultaneous elution and concentration of proteins and antibodies, and an exchange tip that regulates flow for controlled, continuous diafiltration.

Features:

  • Flexibility: designed for simultaneous antibody and protein labelling, purification, sample concentration, or controlled diafiltration
  • Improved recovery: eliminates sample transfer steps for decreased protein loss
  • Time savings: combines multiple protein workflow processes in a single device

Amicon® Stirred Cells

Amicon® Stirred Cells use pressurized ultrafiltration (pUF) at high flow rates to process large sample volumes for rapid concentration, diafiltration, and buffer exchange. Gas pressure is applied directly to the cell for filtration. Compatible membrane filters include Ultracel® RC (regenerated cellulose) and Biomax® PES (polyethersulfone) membranes, offered in a broad range of nominal molecular weight limits ranging from 1 kDa to 500 kDa. Solutes above the membrane’s nominal molecular weight cut-off (NMWCO) are retained in cell, while water and solutes below the cut-off pass into the filtrate and out of cell.

Features:

  • Large volume ultrafiltration: offered in three different sizes to handle volumes from 50 mL to 400 mL, with an optional external 800 mL reservoir for expanded capacity
  • Gentle sample concentration: magnetic stirring minimizes concentration polarization and shear denaturation
  • Rapid ultrafiltration: high flow rates for solutions up to 10% macrosolute concentration
  • Reusable and autoclavable: Amicon® Stirred Cells can be autoclaved for sterility
Related Product Categories
Laboratory Concentration Devices
Centrifugal and pressurized ultrafiltration devices for high recovery protein concentration and concentration of other biological and environmental samples in preparation for downstream applications.
Laboratory Dialysis & Diafiltration Devices
Dialyzers, centrifugal ultrafiltration devices, and stirred cells for buffer exchange, dialysis, desalting, and diafiltration of 10 µL – 1,200 mL sample volumes.
Protein Electrophoresis Gels & Buffers
Precast polyacrylamide protein gels, running buffers, and hand-casting reagents for hand-pouring polyacrylamide gels for PAGE and SDS-PAGE protein gel electrophoresis.
Recombinant/Fusion Tag Protein Purification
Optimize protein purification with our range of affinity resins and buffers for multiple tags and purification techniques.
Antibody Protein Purification
Antibody purification resins and prepacked columns for lab scale, process scale, and industry scale workflows.
Biotinylated Protein Purification
Streptavidin and avidin resins and prepacked columns for small- to large-scale purification of biotinylated proteins and antibodies.
FLAG Purification
Expression vectors, affinity resins, and antibodies for expressing, purifying, and detecting FLAG® tagged fusion proteins.
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Protein Purification
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Protein Labeling & Modification
Protein labeling applications using enzyme-protein labeling, fluorescent protein labeling, and targeted protein degradation technologies.
Protein Mass Spectrometry
An overview of techniques for protein mass spectrometry used in the identification and quantification of proteins, post-translational modifications, glycan profiling, and protein-protein interactions in drug discovery and proteomics research.

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