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Coupling Reagents & Nucleosides

Example reaction scheme for oligonucleotide synthesis showing the deblocking, coupling, capping, and oxidation

We offer a collection of useful building blocks, coupling reagents, and nucleosides for applications in oligo synthesis and beyond. Nucleosides and modified nucleosides are the basic structural units that make up nucleic acids (DNA and RNA), which are responsible for encoding, transmitting and expressing genetic information in all living things. Oligonucleotide synthesis outside of biological systems is the chemical synthesis of nucleic acids of a predetermined sequence that may contain nucleosides beyond those that are naturally occurring. Typical oligonucleotide synthesis using nucleoside phosphoramidites occurs in a four-step process: deblocking, coupling, capping, and oxidation. Coupling reagents play an important role in these steps, efficient coupling activators assuring the reactions are fast and nearly quantitative.

The ability to incorporate non-natural nucleosides into nucleic acids has led to many research uses such as:

  • sequence detection
  • hydrolysis resistance
  • sequencing
  • enzyme inhibition

Nucleoside chemistry has produced non-natural analogs that have been useful in medicine with chemotherapeutic and antiviral applications, such as reverse transcriptase inhibition. Our selection of unnatural bases, nucleoside derivatives and intermediates will provide you with a useful starting point for your oligo or nucleoside analog synthesis needs.

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