P300-05

Porcine Coronary Artery Endothelial: PCAEC (Cryovial)

• UNSPSC Code: 41106514
• NACRES: NA.81

Properties

• biological source: Porcine coronary artery
• Quality Level: 100
• form: solid
• packaging: pkg of 500,000 cells
• manufacturer/tradename: Cell Applications, Inc
• growth mode: Adherent
• karyotype: 2n = 38
• morphology: Endothelial
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cardiovascular diseases
• shipped in: dry ice
• storage temp.: −196°C

Description

General description

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Arterial endothelial cells play critical roles in cardiac homeostasis, and insults to the endothelial cells have been linked to a variety of vascular diseases. Porcine artery endothelial cells provide a useful tool to study artery endothelial function and metabolism.PCAEC from Cell Applications, Inc. have been used to investigate a new mechanism of obesity-associated vascular disease by demonstrating that resistin, an adipocyte-derived cytokine implemented in insulin resistance and diabetes, can cause endothelial dysfunction in coronary arteries through oxidative stress and down-regulation of NO synthase (Kougias, 2005). Conversely, secretoneurin was shown to activate expression of NO synthase and calmodulin, leading to endothelial relaxation (Chan, 2011). PCAEC were also used in adhesion and transmigration experiments in a study investigating anti-inflammatory, cardioprotective effects of a novel adenosine-lidocaine drug formulation (Shi, 2012).

Cell Line Origin

Artery

Application

cardiovascular function, angiogenesis, vascular biology, regulation of coronary blood flow, cardiac functions, effects of vaso-dilators, coagulation, fibrinolysis, vessel wall tone, cytokine production

Components

Porcine Endothelial Cell Growth Medium that contains 10% FBS and 10% DMSO

Preparation Note

• 2nd passage, >500,000 cells in Porcine Endothelial Cell Growth Medium that contains 10% FBS and 10% DMSO
• Can be cultured at least 12 doublings

Subculture Routine

Please refer to the PCAEC Culture Protocol.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable