H6136
Hanks′ Balanced Salts
Without sodium bicarbonate, powder, suitable for cell culture
Pharma Manufacturing
Synonym(s):
Hanks′ Balanced Salt Solution
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General description
Balanced Salt solutions (BSS) are essential in cell culture as they serve as irrigating, transporting, and diluting agents to maintain the osmotic balance between intra-and extracellular spaces. They provide cells with water, inorganic ions, and energy for normal cell metabolism. BSS also provides a buffering system to maintain the medium within the optimum pH range (7.2-7.6).
Application
Hanks′ Balanced Salt has been used:
- in the preparation of stock solution for culturing mice primary hepatocytes
- to measure the mineralized nodule formation in human osteoblast cultures
- in the preparation of embryo buffer to collect zebrafish eggs
Quantity
Formulated to contain 9.8 grams of powder per liter of medium.
Reconstitution
Supplement with 0.35 g/L sodium bicarbonate.
Other Notes
The pH is adjusted after addition of sodium bicarbonate.
supplement
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Protocols
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
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