MFE-296

98031101, human endometrium, Epithelial

• Synonym(s): MFE296 Cells
• UNSPSC Code: 41106514

Properties

• product name: MFE-296, 98031101
• biological source: human endometrium
• growth mode: Adherent
• karyotype: Near Tetraploid
• morphology: Epithelial
• products: Not specified
• receptors: Androgen receptor
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human caucasian endometrial adenocarcinoma

Cell Line Description

MFE-296 was obtained from a moderately differentiated human endometrial adenocarcinoma. The cells were shown to express functionally intact androgen receptors. Proliferation was inhibted by progestin R5020 and dihydrotestosterone (DHT), the latter one being antagonised by the antiandrogen Casodex. MFE-296 express vimentin and the cytokeratins 7, 8, 18 and 19. Karyotype analysis revealed near tetraploidy for most cells. Further more, the cells have been shown to be tumorigenic in nude mice.

Application

Tumour biology studies, receptor analysis

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 8,13
D16S539: 11,14
D5S818: 11,13
D7S820: 8,10
THO1: 6,9.3
TPOX: 8
vWA: 14,18

Culture Medium

MEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 i.e. seeding 1-2 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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