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Key Documents

HEL92.1.7

92111706, human blood, Lymphoblast

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About This Item

UNSPSC Code:
41106514

product name

HEL92.1.7, 92111706

biological source

human blood

growth mode

Suspension

karyotype

Not specified

morphology

Lymphoblast

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Human Caucasian erythroleukaemia

Cell Line Description

Derived from 30 year old male Caucasian. Expresses ß-2 microglobulin and Ia antigen. Used in the study of erythroid cell differentiation and globin gene expression. Analogous to Friend cell leukaemia in mice. K-562 has similar characteristics.

Application

Erythroid cell differentiation studies, induced globin synthesis

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10
D13S317: 9,11
D16S539: 11
D5S818: 11
D7S820: 7
THO1: 7
TPOX: 11
vWA: 14,17

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Cell growth can be very slow on resuscitation. Stand the culture flask up overnight using 20% FBS.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

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