C-12072
Human Follicle Dermal Papilla Cells (HFDPC)
500,000 proliferating cells
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About This Item
UNSPSC Code:
41106514
NACRES:
NA.81
Recommended Products
biological source
human dermis
packaging
pkg of 500,000 cells
morphology
( dermal)
technique(s)
cell culture | mammalian: suitable
shipped in
ambient
storage temp.
room temp
General description
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Cell Line Origin
Skin
Application
Primary Human Follicle Dermal Papilla Cells (HFDPC) are isolated mainly from human dermis of the scalp, originating from the occipital or temple region, and are provided in a proliferating format. Information on donor hair and skin color is available on request. HFDPC stain positive for alkaline phosphatase.Dermal papillae are embedded in a laminin and collagen IV rich extracellular matrix at the base of the hair follicles. They are essential for the induction and maintenance of hair growth. Since they have androgen receptors, they can be used for in vitro screening of androgen blocking reagents.
Quality
Rigid quality control tests are performed for each lot of Follicle Dermal Papilla Cells. They are tested for cell morphology, adherence rate, and cell viability. Immunohistochemical tests for significant markers, e.g. alkaline phosphatase, are carried out for each lot. Growth performance is tested through multiple passages up to 10 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, and microbial contaminants (fungi, bacteria, and mycoplasma).
Warning
Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.
Subculture Routine
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Other Notes
Recommended Plating Density: 5000 - 10000 cells per cm2Passage After Thawing: P2Tested Markers: Alkaline phosphatase positiveGuaranteed population doublings: >10
Recommended products
Recommended Primary Cell Culture Media:Link
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Articles
Primary human follicle dermal papilla cells and optimized culture medium. Protocols for handling, cell passaging, media, and product use.
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