P2850
Phosphatase Inhibitor Cocktail 1
DMSO solution, mixture of inhibitors
Synonym(s):
Phosphatase Inhibitor
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About This Item
Product Name
Phosphatase Inhibitor Cocktail 1, DMSO solution
form
DMSO solution
Quality Level
shipped in
wet ice
storage temp.
2-8°C
General description
Phosphatase Inhibitor Cocktail 1 includes a mixture of inhibitors that will inhibit the L-isozymes of alkaline phosphatase as well as serine/threonine protein phosphatases such as PP1 and PP2A. This product contains microcystinLR, cantharidin, and (−)-p-bromotetramisole. Phosphatase Inhibitor Cocktail 1 has been replaced with P0044.
Application
Phosphatase Inhibitor Cocktail 1 has been used in nuclear protein isolation and electrophoretic mobility shift assay (EMSA) for catenin activation. It has also been used in western blot analysis.
Biochem/physiol Actions
Phosphatases are active regulators of a number of important biological processes. Inhibition of phosphatases by small molecules is useful to study its biological role.
Quantity
Recommended usage is a 100-fold dilution.
Analysis Note
Tested on cell extracts from animal tissues such as bovine liver, human placenta, and rabbit muscle, as well as on extracts from A431 and Jurkat cell lines.
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
188.6 °F
Flash Point(C)
87 °C
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