135R-1

CD35 (EP197) Rabbit Monoclonal Primary Antibody

• Synonym(s): C3-binding protein, C3BR, C3b/C4b receptor, C4BR, CD35 antigen, CR1, Complement Receptor 1, Complement component (3b/4b) receptor 1 (Knops blood group), Knops blood group antigen
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• Quality Level: 100; 500
• conjugate: unconjugated
• antibody form: culture supernatant
• antibody product type: primary antibodies
• clone: EP197, monoclonal
• description: For In Vitro Diagnostic Use in Select Regions (See Chart)
• form: buffered aqueous solution
• species reactivity: human
• packaging: vial of 0.1 mL concentrate (135R-14); vial of 0.1 mL concentrate Research Use Only (135R-14-RUO); vial of 0.5 mL concentrate (135R-15); vial of 1.0 mL concentrate (135R-16); vial of 1.0 mL concentrate Research Use Only (135R-16-RUO); vial of 1.0 mL pre-dilute Research Use Only (135R-17-RUO); vial of 1.0 mL predilute ready-to-use (135R-17); vial of 7.0 mL pre-dilute ready-to-use Research Use Only (135R-18-RUO); vial of 7.0 mL predilute ready-to-use (135R-18)
• manufacturer/tradename: Cell Marque^™
• concentration: 0.1-5.0 μg/mL (predilute); 2.5-125 μg/mL (concentrate)
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:25-1:100 (concentrated)
• isotype: IgG
• control: tonsil
• shipped in: wet ice
• storage temp.: 2-8°C
• visualization: membranous
• Gene Information: human ... CR1(1378)

Description

General description

CD35 (complement receptor 1) is a cell membrane-bound, monomeric glycoprotein found on numerous cell types including erythrocytes, leukocytes, glomerular podocytes, and follicular dendritic cells. The primary function of CD35 is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. The Knops blood group system is a system of antigens located on this protein. The protein mediates cellular binding to particles and immune complexes that have activated complement. Follicular dendritic cells (FDC) are restricted to the B-cell regions of secondary lymphoid follicles. CD35 antigen is found on erythrocytes, B cells, a subset of T cells, monocytes, as well as eosinophils and neutrophils. Anti-CD35 is considered a mature B-cell marker which labels follicular dendritic reticulum cells and tumors derived from such cells such as follicular dendritic cell tumor/sarcoma.

Quality

United States - IVD
Canada - IVD
European Union - IVD
Japan - RUO

Linkage

CD35 Positive Control Slides, Product No. 135S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

Physical form

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

Preparation Note

Download the IFU specific to your product lot and format
Note: This requires a keycode which can be found on your packaging or product label.

Download the latest released IFU
Note: This IFU may not apply to your specific product lot.

Other Notes

For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com

Legal Information

Cell Marque is a trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable