MABS1933

Anti-METTL3 Antibody, clone 29C8

• Synonym(s): EC:2.1.1.348, MT-A70, Methyltransferase-like protein 3, N6-adenosine-methyltransferase 70 kDa subunit, N6-adenosine-methyltransferase catalytic subunit, hMETTL3
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 29C8, monoclonal
• mol wt: calculated mol wt 64.47 kDa; observed mol wt ~65 kDa
• purified by: using protein G
• species reactivity: human
• species reactivity (predicted by homology): bovine, porcine, canine, monkey, mouse
• packaging: antibody small pack of 100
• technique(s): ELISA: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG2cκ
• epitope sequence: N-terminal
• Protein ID accession no.: NP_062826
• UniProt accession no.: Q86U44
• storage temp.: 2-8°C
• Gene Information: human ... METTL3(56339)

Description

Specificity

Clone 29C8 is a mouse monoclonal antibody that detects METTL3. It targets an epitope within 15 amino acids from the N-terminal region.

Immunogen

Ovalbumin-conjugated linear peptide corresponding to 15 amino acid from the N-terminal region of human METTL3.

Application

Quality Control Testing

Evaluated by Western Blotting in lysate from wild-type C643 cells.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected METTL3 in lysate from wild-type C643 cells, but not in lysate from METTL3 knockout C643 cells.

Tested Applications

Immunoprecipitation Analysis: A representative lot immunoprecipitated METTL3 in Immunoprecipitation applications (Scholler, E., et al. (2018). RNA. 24(4):499-512).

Western Blotting Analysis: A representative lot detected METTL3 in Western Blotting applications (Scholler, E., et al. (2018). RNA. 24(4):499-512).

ELISA Analysis: A representative lot detected METTL3 in ELISA applications (Scholler, E., et al. (2018). RNA. 24(4):499-512).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

N6-adenosine-methyltransferase catalytic subunit (UniProt: Q86U44; also known as EC:2.1.1.348, Methyltransferase-like protein 3, hMETTL3, N6-adenosine-methyltransferase 70 kDa subunit, MT-A70) is encoded by the METTL3 (also known as MTA70) gene (Gene ID: 56339) in human. METTL3 heterodimerizes with METTL14 to form an antiparallel heterodimer, which constitutes an active methyltransferase that methylates adenosine residues at the N6 position of some RNAs and regulates various cellular processes, including differentiation of embryonic and hematopoietic stem cells, cortical neurogenesis, differentiation of T-cells, and primary miRNA processing. In response to UV caused DNA damage, METTL3 rapidly catalyzes the formation of m6A on poly(A) transcripts at DNA damage sites that leads to the recruitment of POLK to these sites. It is localized both in the cytoplasm and in the nucleus. It colocalizes with speckles in interphase nuclei. In response to UV irradiation, it is shown to colocalize to DNA damage sites. Its methyltransferase activity is inhibited by sumoylation. However, sumoylation does not affect its subcellular location or interaction with METTL14. Overexpression of METTL3 is reported in several human cancers and its knockdown is known to promote apoptosis. METTL3 is reported to be strongly and directly phosphorylated by ERK2 at serine 43, 50, and 525 and to a lesser extent by p38 and JNK. METTL3 phosphorylation increases its USP5-mediated deubiquitination that leads to its stabilization. Phosphorylation of METTL3 is reported to promote murine embryonic stem cell differentiation. Clone 29C8 is suitable for complex purification of both METTL3 and-14 and is useful in simultaneous analysis of both proteins. (Ref.: Sun, H-L., et al. (2020). Mol. Cell. 80(4); 633-647; Schöller, E., et al. (2018). RNA 24(4); 499-512; Wang, P., et al. (2016). Mol. Cell. 63(2); 306-317).

Physical form

Purified mouse monoclonal antibody IgG2c in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Reconstitution

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable