AB16527
Anti-MyD88 Antibody, CT
Chemicon®, from rabbit
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, human
manufacturer/tradename
Chemicon®
technique(s)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... MYD88(4615)
Related Categories
Specificity
The pro-inflammatory cytokine IL-1 induced cellular response requires IL-1 receptor complex including IL-1RI and IL-RAcP. Recently, MyD88 was identified as an adapter molecule in the IL-1 signaling pathway (Muzio et al. 1997). MyD88 associates with and recruits IRAK to the IL-1 receptor complex in response to IL-1 treatment and dominant negative form of MyD88 attenuates IL-1R mediated NF-kB activation. MyD88 is also employed as a regulator molecule by IL-18 receptor and human Toll receptor (Adachi et al. 1998; Medzhitov et al. 1998), which are members in the Toll/IL-1R family of receptors. Targeted disruption of the MyD88 gene results in loss of cellular responses to IL-1 and IL-18, and MyD88-defecient mice lack responses to bacterial product LPS that employs Toll-like receptors 2 and 4 (TLR2 and TLR4) as the signaling receptors. MyD88 is a general adapter protein for the Toll/1R family of receptors and plays an important role in the inflammatory response induced by cytokines IL-1 and IL-18 and endotoxin. MyD88 gene is expressed in many tissues.
Immunogen
Epitope: C-terminus
Rabbit anti-MyD88 (C-terminus) polyclonal antibody was raised against a peptide corresponding to amino acids 279 to 296 of human MyD88 (Bonnert et al. 1997), which are identical to those of mouse.
Application
Anti-MyD88 Antibody, C-terminus is an antibody against MyD88 for use in WB.
Research Category
Inflammation & Immunology
Inflammation & Immunology
Research Sub Category
Inflammation & Autoimmune Mechanisms
Inflammation & Autoimmune Mechanisms
Western blot: 1:500 to 1:1000
Whole cell lysate from Jurkat cells can be used as positive control and a 35 kDa band should be detected.
Optimal working dilutions must be determined by end user.
Whole cell lysate from Jurkat cells can be used as positive control and a 35 kDa band should be detected.
Optimal working dilutions must be determined by end user.
Physical form
Format: Purified
Purified immunoglobulin. Liquid in PBS containing 0.02% sodium azide.
Storage and Stability
Maintain at 2-8°C in undiluted aliquots for up to 12 months.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Naturally occurring mutation affecting the MyD88-binding site of TNFRSF13B impairs triggering of class switch recombination.
Almejun, MB; Cols, M; Zelazko, M; Oleastro, M; Cerutti, A; Oppezzo, P; Cunningham-Rundles et al.
European Journal of Immunology null
Ramesh K Paidi et al.
Journal of immunology (Baltimore, Md. : 1950), 207(10), 2521-2533 (2021-10-15)
Many patients with coronavirus disease 2019 in intensive care units suffer from cytokine storm. Although anti-inflammatory therapies are available to treat the problem, very often, these treatments cause immunosuppression. Because angiotensin-converting enzyme 2 (ACE2) on host cells serves as the
MyD88 is an adaptor protein in the hToll/IL-1 receptor family signaling pathways.
Medzhitov, R, et al.
Molecular Cell, 2, 253-258 (1998)
O Adachi et al.
Immunity, 9(1), 143-150 (1998-08-11)
MyD88, originally isolated as a myeloid differentiation primary response gene, is shown to act as an adaptor in interleukin-1 (IL-1) signaling by interacting with both the IL-1 receptor complex and IL-1 receptor-associated kinase (IRAK). Mice generated by gene targeting to
The transmembrane activator TACI triggers immunoglobulin class switching by activating B cells through the adaptor MyD88.
He, B; Santamaria, R; Xu, W; Cols, M; Chen, K; Puga, I; Shan, M; Xiong, H; Bussel et al.
Nature Immunology null
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