HPLC Analysis of Toluene, Uracil and Cytosine on Chromolith^® Performance Diol 100-4.6mm

column

Chromolith^® Performance DIOL, 100 x 4.6 mm (1.53172.0001)

mobile phase

[A] acetonitrile; [B] 5mM Ammoniumacetate buffer pH=6.8; (80:20, A:B)

flow rate

0.5 mL/min

pressure

87 psi (6 Bar)

column temp.

23 °C

detector

254 nm

injection

10 μL

sample

Toluene 0,67mg/ml, Uracil 0,04mg/ml, Cytosine 0,06mg/ml (Diluent: Mobile phase)