技術
HPLC: suitable
測試參數
column: Ascentis Express C18, 10 cm x 3 mm I.D., 2.7 μm particles (53814-U)
mobile phase: [A] water with 0.1% formic acid; [B] methanol
gradient: held at 35% B for 3 min; 35 to 45% B in 10 min; held at 45% B for 2 min; 45 to 100% B in 5 min
flow rate: 0.6 mL/min
pressure: 4105 psi (283 bar)
column temp.: 35 °C
detector: UV, 254 nm
injection: 20 μL
sample: 20 μg/mL in 80:20, water:methanol
適合性
application for HPLC
應用
vitamins, nutraceuticals, and natural products
分析報告
This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of milk thistle related compounds.
法律資訊
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
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