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Merck

SRP6105

Sigma-Aldrich

MDH 来源于细菌

recombinant, expressed in E. coli, ≥95% (SDS-PAGE)

别名:

ECK3225, JW3205, Malate dehydrogenase

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About This Item

分類程式碼代碼:
12352204
NACRES:
NA.32

生物源

bacterial

重組細胞

expressed in E. coli

化驗

≥95% (SDS-PAGE)

形狀

liquid

分子量

34.9 kDa (336 aa, 1-312 aa + NT His Tag)

包裝

pkg of 100 μg

技術

activity assay: suitable

UniProt登錄號

運輸包裝

dry ice

儲存溫度

−70°C

基因資訊

Escherichia coli ... MDH(947854)

一般說明

Malate dehydrogenase (MDH) belongs to the LDH/MDH superfamily and MDH type 1 family. It exists as a homodimer and tetramer in prokaryotes.

生化/生理作用

Malate dehydrogenase (MDH) catalyzes the conversion of malate into oxaloacetate (using NAD+) and vice versa (reversible reaction). This reaction is part of many metabolic pathways, including the citric acid cycle. MDH has a key role in the central oxidative pathway. Malate dehydrogenase is also involved in gluconeogenesis, the synthesis of glucose from smaller molecules.

外觀

1 mg/mL solution in 20 mM Tris-HCl (pH 8.0) containing 1 mM DTT, 50 mM NaCl and 10% glycerol.

準備報告

Centrifuge the vial prior to opening.

其他說明

MGSSHHHHHH SSGLVPRGSH MGSHMKVAVL GAAGGIGQAL ALLLKTQLPS GSELSLYDIA PVTPGVAVDL SHIPTAVKIK GFSGEDATPA LEGADVVLIS AGVARKPGMD RSDLFNVNAG IVKNLVQQVA KTCPKACIGI ITNPVNTTVA IAAEVLKKAG VYDKNKLFGV TTLDIIRSNT FVAELKGKQP GEVEVPVIGG HSGVTILPLL SQVPGVSFTE QEVADLTKRI QNAGTEVVEA KAGGGSATLS MGQAAARFGL SLVRALQGEQ GVVECAYVEG DGQYARFFSQ PLLLGKNGVE ERKSIGTLSA FEQNALEGML DTLKKDIALG EEFVNK

象形圖

Exclamation mark

訊號詞

Warning

危險聲明

危險分類

Eye Irrit. 2

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Function, kinetic properties, crystallization, and regulation of microbial malate dehydrogenase*
Toshiko T
Journal of Zhejiang University. Science. B, 17(4), 247-261 (2016)
Priscila Pini Zenatti et al.
EBioMedicine, 30, 158-166 (2018-03-20)
The drug l-asparaginase is a cornerstone in the treatment of acute lymphoblastic leukemia (ALL). The native E. colil-asparaginase used in Brazil until recently has been manufactured by Medac/Kyowa. Then a decision was taken by the Ministry of Health in 2017

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