SRP0320

JMJD2b active human

recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE)

• 别名: KDM4B, lysine (K)-specific demethylase 4B
• 分類程式碼代碼: 12352200
• NACRES: NA.32

属性

• 生物源: human
• 重組細胞: expressed in baculovirus infected Sf9 cells
• 化驗: ≥70% (SDS-PAGE)
• 形狀: aqueous solution
• 分子量: 83 kDa
• 包裝: pkg of 100 μg
• NCBI登錄號: NM_015015
• UniProt登錄號: O94953
• 運輸包裝: dry ice
• 儲存溫度: −70°C
• 基因資訊: human ... KDM4B(23030)

说明

一般說明

Human JMJD2B, also known as JHDM3B and KDM4B (GenBank Accession No. NM_015015), amino acids 2-500 with N-terminal GST-tag, MW=83 kDa, expressed in Sf9 cells using a Baculovirus expression system.

特異性

0.32 pmole/min/μg Assay conditions: 10μl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 50 mM NaCl, 1mM TCEP, 500 μM a-ketoglutarate, 25 μM iron, 2mM ascorbic acid, 0.01% Tween20, 0.5 μM biotinylated peptide substrate, and JMJD2B (2 – 10ng) added to the wells. Add antibody against di-methylated K9 peptide. Incubate for 30 min, then Streptavidin conjugated secondary antibody followed by Alpha Screening detection.

應用

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

外觀

Formulated in: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% Glycerol.

儲存和穩定性

At least 6 months at –80°C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).

安全信息

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable