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生物源
human
重組細胞
expressed in baculovirus infected insect cells
化驗
≥90% (SDS-PAGE)
形狀
aqueous solution
分子量
75 kDa
包裝
pkg of 20 μg
儲存條件
avoid repeated freeze/thaw cycles
濃度
>0.02 mg/mL
NCBI登錄號
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−70°C
基因資訊
human ... PTPRC(5788)
一般說明
Protein tyrosine phosphatase, receptor type C (PTPRC) or leucocyte common antigen CD45 is expressed on T-cells and is found in multiple isoforms. The extracellular domain of the protein is predicted to have an amino-terminal mucin-like segment, a cysteine-rich domain and three fibronectin type 3 domains. The gene encoding this protein is localized on human chromosome 1q31.3.
Protein tyrosine phosphatase. Catalytic domain, (584-1256) (Gene bank accession number NM_080922) with N-terminal His tag, MW = 75 kDa, expressed in a Baculovirus Sf9 expression system.
應用
Useful for the study of enzyme kinetics, regulation, and to dephosphorylate target substrates.
生化／生理作用
Protein tyrosine phosphatase, receptor type C (PTPRC) or leucocyte common antigen CD45 acts as a protein tyrosine phosphatase in leucocyte signaling. It has a role in flow cytometric diagnostics in leukemia. By dephosphorylation of Src kinases, the protein acts as a regulator of antigen receptor signaling in T and B cells. As a negative regulator of Janus kinases, CD45 inhibits cytokine receptor signaling.
單位定義
One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.
外觀
Formulation: recombinant proteins were eluted in 25 mM Tris-HCL, 75 mM NaCl, pH 8.0, 0.05% Tween, 5 mM DTT and 50% glycerol.
準備報告
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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