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生物源
rabbit
品質等級
共軛
unconjugated
抗體表格
affinity isolated antibody
抗體產品種類
primary antibodies
無性繁殖
polyclonal
形狀
buffered aqueous solution
分子量
antigen ~110 kDa
物種活性
mouse
包裝
antibody small pack of 25 μL
技術
indirect immunofluorescence: 1:50 using mouse NIH3T3 cells
microarray: suitable
western blot: 1:2,000 using mouse NIH/3T3 cells
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
mouse ... Sirt1(93759)
一般說明
Sir2 belongs to a family of proteins that is found in organisms ranging from bacteria to complex eukaryotes. Members of this family contain a 250 amino acid core domain that shares about 25-60% sequence identity.
特異性
Anti-Sir2 polyclonal antibody specifically recognizes mouse Sir2 (AS-16). Staining of the Sir2 band is specifically inhibited by the immunizing peptide.
免疫原
synthetic peptide corresponding to mouse Sir2 sequence (amino acids 722-737 with N-terminal added lysine), conjugated to KLH. This sequence is 62% homologous to the corresponding human sequence.
應用
Anti-Sir2 (AS-16) antibody produced in rabbit has been used in
- western blotting
- immunoprecipitation
- antibody microarray
生化／生理作用
Sir2, one of the silent information regulator genes, encodes a protein that promotes a compact chromatin structure, thereby preventing or silencing gene transcription at selected loci. Silencing occurs as a series of events initiated by formation of Sir complexes (Sir2, Sir3, Sir4). Sir2 protein is an NAD-dependent histone deacetylase. Sir2 transfers acetyl groups from its protein substrates to ADP-ribose and synthesizes o-acetyl ADP-ribose. Through histone deacetylation, Sir2 may silence chromatin. It appears that Sir2 NAD requirement makes this protein an important player in the pathway that leads to increased life span of several species through calorie restriction.
外觀
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Phosphorylation regulates SIRT1 function
Sasaki T, et al.
Testing, 3(12), e4020-e4020 (2008)
Sir2 links chromatin silencing, metabolism, and aging
Guarente L
Genes & Development, 14(9), 1021-1026 (2000)
Vladimir Ljubicic et al.
Aging, 1(9), 818-830 (2010-02-17)
Our intent was to investigate the mechanisms driving the adaptive potential of subsarcolemmal (SS) and intermyofibrillar (IMF) mitochondria in young (6 mo) and senescent (36 mo) animals in response to a potent stimulus for organelle biogenesis. We employed chronic electrical
Progressive loss of SIRT1 with cell cycle withdrawal
Sasaki T, et al.
Aging Cell, 5(5), 413-422 (2006)
Induction of hypothalamic Sirt1 leads to cessation of feeding via agouti-related peptide
Sasaki T, et al.
Endocrinology, 151(6), 2556-2566 (2010)
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