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生物源
rabbit
共軛
unconjugated
抗體表格
whole antiserum
抗體產品種類
primary antibodies
無性繁殖
polyclonal
包含
15 mM sodium azide
物種活性
rat
技術
dot blot: 1:50,000
microarray: suitable
western blot: 1:10,000 using rat brain extract
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
rat ... Prkcd(170538)
一般說明
Protein Kinase C (PKC) is a family of serine/threonine (Ser/Thr)-specific protein kinases. PKC is a phospholipid-dependent enzyme, activated by the lipid 1,2-diacylglycerol (DAG). The protein kinase C delta (PKC δ) isoenzyme appears to be widely expressed in the brain, lung, heart, spleen, liver, ovary, pancreas, thymus, adrenal gland, skin and rat embryonic fibroblasts, and is expressed in lower levels in certain mouse fibroblasts. PKD is also located in the cytosol, nuclear compartment and in mitochondria in response to cellular stress.
特異性
Anti-Protein Kinase C δ specifically reacts in dot-blot immunoassay with PKC δ peptide conjugated to BSA with 1-ethyl-3-(3-dimethylamino-propyl)-carbodiimide (EDCI).
免疫原
Synthetic peptide corresponding to the C-terminal variable (V5) region (amino acids 662-673) of rat PKC δ coupled to KLH with glutaraldehyde.
應用
Anti- protein kinase c δ antibody may be used in:
- immunoprecipitation
- immunohistochemistry
- immunoblotting
- ELISA
- chemiluminescence detection systems to detect PKC δ
- dot-blot immunoassay
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
生化／生理作用
Protein Kinase C isotype δ (PKCδ) modulates the inflammatory response. It acts as a signal transducer of several signaling pathways. Hence it can be considered as a vital therapeutic target to treat sepsis induced-lung injury. In sepsis, PKCδ participates in platelet-mediated activation. Overexpression and stimulation of PKC δ leads to cell division arrest in Chinese hamster ovary (CHO) cells and growth inhibition of NIH3T3 cells.
Protein kinase C (PKC) has a pivotal role in cell growth and differentiation, modulation of neurotransmission, signal transduction and oncogenesis. Anti-protein kinase c δ antibody can be used for studying the differential tissue expression and intracellular localization of PKC δ. It can also be used in western blotting and microarray.
外觀
Rabbit Anti-Protein Kinase C δ is supplied as liquid containing 0.1% sodium azide as preservative.
儲存和穩定性
For continuous use, store at 2-8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended.Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Sean M Crosson et al.
Molecular therapy. Methods & clinical development, 10, 1-7 (2018-08-04)
Adeno-associated virus (AAV) is one of the most promising gene therapy vectors and is widely used as a gene delivery vehicle for basic research. As AAV continues to become the vector of choice, it is increasingly important for new researchers
Manuela Cerezo et al.
European journal of pharmacology, 522(1-3), 9-19 (2005-10-06)
The present study was designed to investigate the possible changes of protein kinase A (PKA) and different isoforms of protein kinase C (PKC): PKC alpha, PKC delta and PKC zeta after naloxone induced morphine withdrawal in the heart. Male rats
Ikuko Koyama-Honda et al.
Autophagy, 9(10), 1491-1499 (2013-07-26)
Autophagosome formation is governed by sequential functions of autophagy-related (ATG) proteins. Although their genetic hierarchy in terms of localization to the autophagosome formation site has been determined, their temporal relationships remain largely unknown. In this study, we comprehensively analyzed the
Protein kinase D activation induces mitochondrial fragmentation and dysfunction in cardiomyocytes
Jhun BS, et al.
The Journal of Physiology, 596(5), 827-855 (2018)
Peidu Jiang et al.
Molecular biology of the cell, 25(8), 1327-1337 (2014-02-21)
Membrane fusion is generally controlled by Rabs, soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs), and tethering complexes. Syntaxin 17 (STX17) was recently identified as the autophagosomal SNARE required for autophagosome-lysosome fusion in mammals and Drosophila. In this study, to better
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