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Merck
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Key Documents

IAK0108

Sigma-Aldrich

Phagocytosis Assay Kit

Green Zymosan, sufficient for 100 fluorometric assays

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.84

適合性

suitable for fluorescence

儲存溫度

2-8°C

一般說明

Phagocytosis is a cellular process for ingesting and eliminating microorganisms, foreign substances, and apoptotic cells. It is a specific form of endocytosis initiated by recognition and binding of foreign particles by cell surface receptors, followed by their engulfment, and formation of phagosomes. Maturing phagosomes transform to phagolysosomes which destroy the pathogen through enzymes and toxic peroxides. Zymosan prepared from yeast cell wall (Saccharomyces cerevisiae) and consisting of protein-carbohydrate complexes is frequently used as a pathogen in phagocytosis assays.

應用

The Phagocytosis Assay Kit (Green Zymosan) uses fluorescently pre-labeled Zymosan particles to detect and quantify in vitro phagocytosis by fluorescent microscope, spectrophotometer (Ex/Em = 490/520 nm) or flow cytometry.

適合性

This kit is suitable for screening activators and/or inhibitors of phagocytosis and Toll-like receptor ligands (TLR) in mammalian cells.

象形圖

Health hazard

訊號詞

Danger

危險聲明

危險分類

Carc. 1B

儲存類別代碼

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects


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