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H1404

Sigma-Aldrich

肝细胞生长因子 人

HGF, recombinant, expressed in Baculovirus infected High-5 cells, suitable for cell culture

别名:

HGF, 散射因子, 肝素A

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About This Item

EC號碼:
214-275-1
MDL號碼:
MFCD00217422
分類程式碼代碼:
12352202
NACRES:
NA.77

生物源

human

品質等級

200

重組細胞

expressed in Baculovirus infected High-5 cells

化驗

>95% (SDS-PAGE)

形狀

lyophilized powder

效力

1.0-20.0 ng/mL

品質

endotoxin tested

分子量

~80 kDa

包裝

pkg of 5x5 μg
pkg of 5 μg

儲存條件

avoid repeated freeze/thaw cycles

技術

cell culture | mammalian: suitable

雜質

<1.0 EU/μg

UniProt登錄號

P14210

儲存溫度

−20°C

基因資訊

human ... HGF(3082)

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相关类别

生化／生理作用

肝细胞生长因子（HGF）是肝细胞和多种细胞类型（包括内皮和上皮细胞、黑素细胞和角质形成细胞）的有效促分裂原。它还可介导上皮形态发生。HGF是由met原癌基因编码的酪氨酸激酶受体的配体。

其他說明

NSO表达的重组HGF含有大于95％的异二聚体HGF。昆虫细胞系表达的重组HGF是单链HGF（主要）和一些异二聚体HGF的混合物。单链HGF和异二聚体HGF在 体外 分析中具有相同的活性。

外觀

从经0.2μm过滤的含有20 mM NaH2PO4 (pH 7.0)和500 mM NaCl,并且每1μg HGF中含50μg BSA的溶液冻干得到

準備報告

在含有至少0.1％人或牛血清白蛋白的无菌PBS中以50μg/mL的浓度进行复溶。复溶后，该溶液可以在2-8°C下最多保存一个月，或在-20°C到-70°C下最多保存三个月。请使用手动除霜冷冻柜，并避免反复冻融。

分析報告

生物学活性通过其诱导Saos-2人骨肉瘤细胞分泌IL-11的能力进行测定。

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 2

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

dust mask type N95 (US), Eyeshields, Gloves

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