GE17-0920-05

HiTrap^® IMAC High Performance

Cytiva 17-0920-05, pack of 5 × 5 mL

• 分類程式碼代碼: 23151816
• NACRES: NA.56

属性

• 儲存期限: Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
• 包裝: pack of 5 × 5 mL
• 製造商／商標名: Cytiva 17-0920-05
• 參數: <20 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.); 42 psi (H₂O at room temperature.)
• 床尺寸: 16 mm × 25 mm
• 床體積: 5 mL
• 柱內徑: 16 mm
• 基質: highly cross-linked 6% agarose
• 樣品平均尺寸: 34 μm
• cleaning: 2-14(Medium uncharged with metal ions.)
• 工作範圍: 3-12
• 容量: ≥40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.)

说明

一般說明

HiTrap^® IMAC HP is prepacked with IMAC Sepharose^™ High Performance. The medium is charged with the metalion of your choice for Immobilized Metal ion Affinity Chromatography (IMAC) and subsequent high-resolution purification of polyhistidine tagged proteins.

應用

IMAC Sepharose^™ HP is supplied free of metal ions. It is charged by the user with the transition metal ion of choice (e.g. Cu2+, Zn2+, Ni2+, or Co2+); these metal ions will bind to the covalently immobilized chelating ligand on the Sepharose.

特點和優勢

• For optimizing purification of histidine-tagged Proteins when Ni2+ is not the best choice of metal ion.
• Conveniently charge with your metal of choice.
• Small bead size for high-performance purification.
• High binding capacity.

儲存和穩定性

Store at 4 to 30 °C (20% Ethanol)

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

IMAC Sepharose products, Ni Sepharose products and Fe Sepharose products
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.

HiTrap is a registered trademark of Cytiva

Sepharose is a trademark of Cytiva

安全信息

• 象形圖: GHS02
• 訊號詞: Warning
• 危險聲明: H226
• 防範說明: P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501
• 儲存類別代碼: 3 - Flammable liquids