GE17-0920-05

HiTrap^® IMAC High Performance

Cytiva 17-0920-05, pack of 5 × 5 mL

• 别名: Metal affinity chromatography
• 分類程式碼代碼: 23151816
• NACRES: NA.56

属性

• 包裝: pack of 5 × 5 mL
• 製造商／商標名: Cytiva 17-0920-05
• 參數: 42 psi (H₂O at room temperature.)
• 床尺寸: 16 mm × 25 mm
• 床體積: 5 mL
• 柱內徑: 16 mm
• 基質: highly cross-linked 6% agarose
• 樣品平均尺寸: 34 μm
• cleaning: 2-14(Medium uncharged with metal ions.)
• 工作範圍: 3-12
• 容量: ≥40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.)

说明

一般說明

HiTrap^® IMAC HP is prepacked with IMAC Sepharose^™ High Performance. The medium is charged with the metalion of your choice for Immobilized Metal ion Affinity Chromatography (IMAC) and subsequent high-resolution purification of polyhistidine tagged proteins.

應用

IMAC Sepharose^™ HP is supplied free of metal ions. It is charged by the user with the transition metal ion of choice (e.g. Cu2+, Zn2+, Ni2+, or Co2+); these metal ions will bind to the covalently immobilized chelating ligand on the Sepharose.

特點和優勢

• For optimizing purification of histidine-tagged Proteins when Ni2+ is not the best choice of metal ion.
• Conveniently charge with your metal of choice.
• Small bead size for high-performance purification.
• High binding capacity.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

其他說明

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法律資訊

IMAC Sepharose products, Ni Sepharose products and Fe Sepharose products
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.

HiTrap is a registered trademark of Cytiva

Sepharose is a trademark of Cytiva

安全信息

• 象形圖: GHS02
• 訊號詞: Warning
• 危險聲明: H226
• 防範說明: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• 儲存類別代碼: 3 - Flammable liquids