COLO 839

93051119, human blood, Lymphoblastoid

• 分類程式碼代碼: 41106514

属性

• product name: COLO 839, 93051119
• 生物源: human blood
• 增長模式: Suspension
• 染色體組型: Not specified
• 形態學: Lymphoblastoid
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human Caucasian B lymphocyte

細胞系描述

Derived from the peripheral blood of a 43 year old Caucasian male with widespread melanoma. The cells were transformed with defective EBV (B-95-W8).
COLO 839 (Sigma Catalogue number 93051119), COLO 858 (Sigma Catalogue number 93052613) and COLO 857 (Sigma Catalogue number 93051120) were all derived from the same 43 year-old male patient with malignant melanoma (see references below). This has been confirmed by STR profiling.

應用

Tumourigenicity studies

DNA分析

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,13
D13S317: 11,12
D16S539: 11,13
D5S818: 11,12
D7S820: 8,10
THO1: 9.3,10
TPOX: 8,11
vWA: 17,19

培養基

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Maintain cultures between 2-9x100,000 cells/ml; 5% CO₂; 37°C.

其他說明

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