L6.C11

92102119, rat muscle, Fibroblast

• 分類程式碼代碼: 41106514

属性

• product name: L6.C11, 92102119
• 生物源: rat muscle
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Fibroblast
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Rat skeletal muscle myoblast

細胞系描述

A subclone of L6, a rat thigh muscle cell line. On reaching confluence the cells will fuse to form myotubes and twitching movement will be seen. Once cultures have fused they cannot be recloned.

培養基

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split semi-confluent cultures to a seeding density of 1.5-2x1,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Cells fuse on reaching confluence, producing myotubes. To prevent fusion maintain the cultures sub-confluent and reclone every 6-8 weeks.

其他說明

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