GS-109-V-34

90110504, human skin, Fibroblast

• 分類程式碼代碼: 41106514

属性

• product name: GS-109-V-34, 90110504
• 生物源: human skin
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Fibroblast
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human Caucasian skin fibroblast Gardner′s syndrome

細胞系描述

Derived from a 6 year old Caucasian female with Gardner′s syndrome, an autosomal dominant condition which predisposes to carcinoma and multiple polyps of the colon. Normal female karyotype.

應用

Tumourigenicity studies

DNA分析

STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 8,11
D16S539: 11,12
D5S818: 12
D7S820: 8,12
THO1: 6,9.3
TPOX: 10,12
vWA: 14,16

培養基

EMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 20% Foetal Bovine Serum (FBS).

例行更新培養

Split semi-confluent cultures 1:2 i.e. seeding at 4x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. Monolayer should form in a 25cm² in 3-4 days.

其他說明

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