GS-109-V-63

90110503, human skin, Fibroblast

• 分類程式碼代碼: 41106514

属性

• product name: GS-109-V-63, 90110503
• 生物源: human skin
• 增長模式: Adherent
• 染色體組型: Modal no. 46
• 形態學: Fibroblast
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human Caucasian skin fibroblast Gardner′s syndrome

細胞系描述

Derived from a 13 year old Caucasian male with Gardner′s syndrome, an autosomal dominant condition which predisposes to carcinoma and multiple polyps of the colon. The modal chromosome number was 46, occurring in 60% of cells.

應用

Tumourigenicity studies

DNA分析

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11
D13S317: 11,12
D16S539: 12,13
D5S818: 11,12
D7S820: 9,10
THO1: 7,8
TPOX: 8,11
vWA: 14,17

培養基

EMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 20% Foetal Bovine Serum (FBS).

例行更新培養

Split semi-confluent cultures 1:2 i.e. seeding at 4x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. Monolayer should form in a 25cm² flask in 3-4 days.

其他說明

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