IgH-2

90030804, lizard cardiovascular , Epithelial-like

• 分類程式碼代碼: 41106514

属性

• 产品名称: IgH-2, 90030804
• 生物源: lizard cardiovascular
• 增長模式: Adherent
• 染色體組型: 2n = 34
• 形態學: Epithelial-like
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Iguana heart

細胞系描述

From the heart of a normal immature male Iguana. Cells can support the replication of herpes simplex, PRV, vaccinia and Iguana viruses at 36°C. The only reported reptilian line to be established and serially propagated at an incubation temperature as high as 36°C.

應用

Virus replication studies: HSV, PRV, vaccinia and Iguana viruses

培養基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 36°C.

其他說明

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