NRK-52E

87012902, rat kidney, Epithelial

• 分類程式碼代碼: 41106514

属性

• product name: NRK-52E, 87012902
• 生物源: rat kidney
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Epithelial
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Rat kidney epithelial

細胞系描述

Cloned from the same mixed culture of normal rat kidney cells as NRK-49F, but has epithelial-like morphology. It has distinct growth and transforming characteristics distinct from NRK-49F.

應用

NRK-52E has been used to study the effects of altered selenium supply on the expression of endogenous thioredoxin reductase (TrxR).

培養基

DMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 5-10% Foetal Bovine Serum (FBS) or New Born Calf Serum (NBCS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Remove all traces of trypsin from cultures during sub-culturing i.e. spin cells or neutralise with serum.

其他說明

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