IIC9 (CH0)

30814, hamster embryo, Fibroblast

• 分類程式碼代碼: 41106514

属性

• product name: IIC9 (CH0), 00030814
• 生物源: hamster embryo
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Fibroblast
• 產品: Not specified
• 受體: PAR-1 (protease-activated receptor 1, thrombin receptor), EGF, PDGF
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Chinese hamster embryo fibroblast

細胞系描述

The Chinese hamster embryo fibroblast (CHEF) line is a nontumorigenic and stable diploid cell line, with a high serum requirement for growth. It is isolated from embryo preparation and is a spontaneous tumorigenic anchorage-independent line.

應用

IIC9 (CH0) is suitable for selective growth of some rodent epithelial cells in a medium containing citrulline.

培養基

DMEM:F12 (1:1) (D8062) + 5% FBS / FCS (F2442) + 2mM L-Glutamine (G7513).

例行更新培養

Split sub-confluent cultures (70-80%) 1:10 ie. seeding at 2-4 x10,000 cells/cm² using 0.25% trypsin /EDTA; 37°C; 5% CO₂.

其他說明

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