生物源
mouse
品質等級
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
MnS-1, monoclonal
物種活性
rat, rabbit, human
應無反應活性
mouse
製造商／商標名
Chemicon®
技術
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
同型
IgG1
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... SOD2(6648)
特異性
Antibody reacts with MnSOD
應用
Immunohistochemistry on acetone fixed cryostat sections: 0.5-10 μg/mL using an APAAP or PAP procedure. Antibody also reacts with 4% PFA fixed frozen tissue. Typical block contains 1% non-fat dry milk or 3% NGS and 0.2-1.5% triton X-100 {higher triton is only used on thick sections; blocked for 1-3 hours at room temperature. Primary antibody is diluted only in PBS or TBS without excess blocking materials (no triton or milk); typical dilution is 1:200-1:1000 with incubations up to 48 hours at 4C. Washed 3x10′ in PBS and detect via DAB. Immunoreactive neurons are characterized by the dark brown, fine granular deposition in soma and proximal dendrites.
Immunocytochemistry on cell smears: 0.5-10 μg/mL.
Western blot: MnS1 antibody will recognize at 25kDa band under reducing conditions; this is the monomer of the MnSOD protein.
Optimal working dilutions must be determined by end user.
Immunocytochemistry on cell smears: 0.5-10 μg/mL.
Western blot: MnS1 antibody will recognize at 25kDa band under reducing conditions; this is the monomer of the MnSOD protein.
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Oxidative Stress
Oxidative Stress
This Anti-MnSOD Antibody, clone MnS-1 is validated for use in WB, IC, IH for the detection of MnSOD.
外觀
Format: Purified
Purified immunoglobulin. Liquid in PBS.
儲存和穩定性
Prior to opening maintain at 2-8°C for up to six month after date of receipt. After opening maintain at -20°C in undiluted aliquots for up to six months. Avoid repeated freeze/thaw cycles.
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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