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Key Documents

MAB1982

Sigma-Aldrich

抗整联蛋白α 6抗体,克隆MA6

clone MA6, Chemicon®, from rat

别名:

CD49f

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

rat

品質等級

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

MA6, monoclonal

物種活性

mouse

製造商/商標名

Chemicon®

技術

flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable

同型

IgG2κ

適合性

not suitable for Western blot

UniProt登錄號

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

human ... ITGA6(3655)

特異性

与层粘连蛋白受体α-β-1和α-β-4 复合物反应。

應用

冰冻组织切片的免疫沉淀、流式细胞术、免疫组织化学。该产品在蛋白质印迹中不可检测α6蛋白,因为该表位最可能涉及α 6 和β 1整联蛋白亚基。该产品不可阻止α6 整联蛋白介导的细胞与层粘连蛋白的粘附。通过MA6 激活的整联蛋白信号转导特性还尚未被表征。以10E6细胞当量的裂解液中进行VLA-6的免疫沉淀需要2 μg MA6。流式细胞术的推荐浓度- 10 μg/mL,冰冻组织切片染色- 20 μg/mL。

最佳工作稀释度必须由最终用户进行确定。

采用MA6 测试表达6种整联蛋白的细胞系包括:小鼠骨髓源性肥大细胞、黑色素瘤细胞系B16F1、鳞状细胞癌SCC-VII、成纤维细胞系NIH-3T3、脾脏T淋巴细胞、胸腺细胞和各种T细胞系。
该抗整联蛋白α6抗体(克隆MA6)经验证可用于FC、IC、IH、IP检测整联蛋白α6。

外觀

形式:纯化
溶于含0.25M NaCl和0.1%叠氮化钠的0.02M磷酸盐缓冲液(pH 7.6)的液体。

儲存和穩定性

可在+2-8°C保存长达12个月。

其他說明

浓度:请参考批次特异性浓缩物的检验报告。

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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