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生物源
mouse
品質等級
共軛
PE
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
LM609, monoclonal
物種活性
monkey, pig, rabbit, bovine, avian, chicken, canine, human
應無反應活性
mouse, rat
製造商／商標名
Chemicon®
技術
flow cytometry: suitable
immunohistochemistry: suitable
同型
IgG1
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... ITGAV(3685)
一般說明
EMISSION MAXIMUM:
580 nm
580 nm
The integrin family of cell adhesion receptors consists of at least 16 membrane-associated heterodimers, composed of an alpha and beta subunit that associate in a non-covalent manner. The structure and functional diversity of the integrin family are based upon the pairing abilities of the individual alpha and beta subunits. Key to these molecular interactions between the integrin receptors and their respective ligands is the recognition of the Arg-Gly-Asp (RGD) sequence, known to be present in the extracellular matrix components fibronectin, vitronectin, collagen, fibrinogen, and von Willebrand factor (Cheresh, 1991). The involvement of integrins in vascular proliferation, adhesion, and wound repair has been well documented. The adhesion receptor, integrin alphaVbeta3, appears to be selectively expressed on growing blood vessels and has been identified as a marker of angiogenic vascular tissue (Brooks, 1994). Due to its involvement in angiogenesis, integrin alphaVbeta3 is one of the most intensely studied of the integrin receptors.
特異性
Monoclonal antibody LM609 is reactive with the vitronectin receptor alphaVbeta3 complex, an RGD-directed adhesion receptor. LM609 has been demonstrated to block adhesion of a human melanoma cell line (M21) to vitronectin, fibrinogen and von Willebrand factor, as well as to a synthetic RGD containing peptide (Cheresh, 1987). In chick chorioallentoic membranes, LM609 was shown to block angiogenesis induced by bFGF and TNFalpha but had no effect on pre-existing vessels (Brooks, 1994). While LM609 does block cell attachment to RGD containing ligands, it does not interact directly with the RGD binding site. Instead, LM609 appears to be an allosteric inhibitor of integrin alphaVbeta3, which binds to a conformational epitope resulting from the post-translational association of the alphaV and beta3 subunits.
ABSORPTION MAXIMA: 90 nm, 545 nm and 565 nm
ABSORPTION MAXIMA: 90 nm, 545 nm and 565 nm
免疫原
Purified adhesion receptor from the human melanoma cell line M21 (Cheresh, 1987).
應用
Anti-Integrin αVβ3 Antibody, clone LM609, Phycoerythrin conjugated detects level of Integrin αVβ3 & has been published & validated for use in FC, IH.
Flow Cytometry: 1-3 μg LM609 per 0.5 x 10e6 cells in 100μL (Smith, 1996).
Immunofluorescence: 5-10 μg/mL for staining of bFGF-treated 6 mm cryosections of chick chorioallantoic membrane fixed with acetone (Brooks, 1994). LM609 is also effective on 4% paraformaldehyde fixed, frozen tissues and cells. However it is not effective for immunohistochemical staining of paraffin-embedded tissue sections.
Optimal working dilutions and protocols must be determined by end user.
Immunofluorescence: 5-10 μg/mL for staining of bFGF-treated 6 mm cryosections of chick chorioallantoic membrane fixed with acetone (Brooks, 1994). LM609 is also effective on 4% paraformaldehyde fixed, frozen tissues and cells. However it is not effective for immunohistochemical staining of paraffin-embedded tissue sections.
Optimal working dilutions and protocols must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Integrins
Integrins
外觀
Liquid in 0.01M sodium phosphate (pH7.6), 0.25 M NaCl, 0.05% sodium azide, 0.01% Thimerosal and 15 mg/mL BSA added as a stabilizer.
儲存和穩定性
Maintain at 2-8°C in undiluted aliquots for up to 6 months. Protect from Light. Do not store as a dilute solution.
分析報告
Control
POSITIVE CONTROL: Human myeloma cell lines M21 and MoalphaV (Chen, 1995).
NEGATIVE CONTROL: Mo cells (this cell line was derived from M21 but does not express integrin alphaV; Chen, 1995).
POSITIVE CONTROL: Human myeloma cell lines M21 and MoalphaV (Chen, 1995).
NEGATIVE CONTROL: Mo cells (this cell line was derived from M21 but does not express integrin alphaV; Chen, 1995).
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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