MAB1527

Anti-Peripherin Antibody, clone 8G2

culture supernatant, clone 8G2, Chemicon^®

• 别名: Anti-NEF4, Anti-PRPH1
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物源: mouse
• 品質等級: 100; 300
• 抗體表格: culture supernatant
• 抗體產品種類: primary antibodies
• 無性繁殖: 8G2, monoclonal
• 物種活性: pig, rat, bovine, human, mouse
• 製造商／商標名: Chemicon^®
• 技術: immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• 同型: IgG
• 適合性: not suitable for immunohistochemistry (Paraffin)
• NCBI登錄號: NM_006262.3
• UniProt登錄號: P41219
• 運輸包裝: dry ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... PRPH2(5961)

说明

一般說明

Peripherin is a ~57kDa intermediate filament subunit found initially in sensory neurons of the peripheral nervous systems. Subsequently it was found in some sensory and other neurons of the central nervous system and also in PC12 cells. Peripherin is also expressed in certain neuroendocrine tumors and in the insulin producing cells of the pancreas. Peripherin belongs to the Class III family of intermediate filament subunits which includes vimentin, glial fibrillary acidic protein (GFAP) and desmin. Antibodies to peripherin can be used in identifying, classifying, and studying neurons throughout the nervous system. Peripherin is also a good diagnostic marker for ballooned axons seen in Lou Gehrig′s disease (Amyotrophic lateral sclerosis) and some neuronally derived tumors. Autoantibodies to peripherin are frequently seen in the sera of patients with diabetes. Peripherin is not related to peripherin/RDS, a protein of the photoreceptor outer membrane mutations of which are causative of certain forms of slow retinal degeneration.

特異性

Recognizes Peripherin. Strong staining on rat, mouse, human, pig and cow peripherin. Does not work on chicken or other more distantly related species, which may lack peripherin.

免疫原

Electrophoretically pure trp-E-peripherin fusion protein [Dev. Brain Res. (1990). 57:235-248] containing all but the 4 N-terminal amino acids of rat peripherin. Fusion protein purified from bacterial inclusion bodies by DEAE-cellulose chromatography in 6M urea followed by preparative SDS-PAGE

應用

Anti-Peripherin Antibody, clone 8G2 is an antibody against Peripherin for use in IC, IH & WB.

Immunocytochemistry: 4% PFA fixed cells (5 minutes) or acetone is recommended.

Immunohistochemisty: light PFA fixation is necessary as 8G2 is sensitive to heavy formalin fixation. Antigen recovery is enhanced with 0.1% triton in the block only. 1:25-1:200

Immunoblotting: The antibody is clean and specific for the expected 57kDa band on Western blots.

Optimal working dilutions must be determined by end user.

Research Category
Neuroscience

Research Sub Category
Sensory & PNS

Neuronal & Glial Markers

標靶描述

57kDa

外觀

Unpurified

UnPurified mouse culture supernatant containing no preservatives.

儲存和穩定性

Maintain for 12 months at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

分析報告

Control
Rat sensory neurons, rat spinal cord homogenate and peripheral nerve homogenate

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable