推荐产品
生物源
guinea pig
品質等級
抗體表格
serum
抗體產品種類
primary antibodies
無性繁殖
polyclonal
物種活性
rat, mouse
技術
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
mouse ... Rbfox3(52897)
rat ... Rbfox3(287847)
一般說明
ABN90是抗NeuN，克隆A60（MAB377）的豚鼠多克隆版本，是高度表征和引用的小鼠单克隆抗体，可特异性识别DNA结合的神经元特异性蛋白NeuN，该蛋白存在于测试的全部脊椎动物的大多数CNS和PNS神经元细胞类型中。NeuN蛋白的分布明显局限于胎儿和成人大脑中的神经元细胞核、核周体和一些近端神经元突起，但有些神经元在所有年龄段都不能被NeuN识别：INL视网膜细胞，Cajal-Retzius细胞，Purkinje细胞，下橄榄和齿状核神经元以及交感神经节细胞就是一些例子。免疫组化检测的NeuN蛋白首先出现在神经元从细胞周期中退出和/或与神经元终末分化开始所对应的发育时间点。免疫反应性出现在小鼠神经管中的E9.5附近，且通过E12.5广泛存在于发育中的神经系统中。强核染色代表核调节蛋白的功能；但是，目前还没有证据表明NeuN蛋白抗原在远端细胞质中是否有功能，或者它是否只是在转运回细胞核之前在远端细胞质中合成的。从纯化的细胞核中分离出的蛋白质与免疫印迹上的全脑提取物之间没有差异。
應用
- 免疫组织化学（石蜡）分析：代表性批次的1:5,000稀释液在小鼠大脑皮层和海马体脑组织切片中检测到NeuN。
- 免疫细胞化学分析：代表性批次的1:500稀释液在大鼠E18皮质细胞中检测到NeuN。
- 注意：实际最佳工作稀释度必须由最终用户确定，因为样本和实验条件可能会因最终用户而有所不同。
豚鼠多克隆抗NeuN抗体（货号ABN90）经过测试可用于蛋白质免疫印迹、免疫细胞化学和免疫组化（石蜡）法检测NeuN蛋白。
品質
通过蛋白质印迹法在小鼠E16脑组织裂解物中进行了评估。 蛋白印迹分析：该抗体的1：2,000稀释液在小鼠E16脑组织裂解液中检测到NeuN。
標靶描述
观察值〜48 kDa
外觀
含0.05%叠氮化钠的豚鼠多克隆血清。
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
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